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The budding of defective human immunodeficiency virus type 1 (HIV-1) particles from cell clones persistently infected with HIV-1

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Summary

Three cell clones producing large numbers of infectious or noninfectious particles of human immunodeficiency virus type 1 (HIV-1), designated M 10/LAV-2, M 16/LAV-3, and MT/LAV-17, were isolated from persistently HIV-1-infected MT-4 cells. In M 10/LAV-2, the HIV-1 proteins were defective in the cleavage ofgag precursor protein, and the particles were doughnutshaped with a double-ring structure. These particles were produced by budding at the cell surface from crescentic structures followed by the formation of double-ring structures. The viral proteins in M 16/LAV-3 were defective in the cleavage ofenv precursor protein. The morphology of the virus particles was intact, and an electron dense bar-shaped core was seen inside a single-ring enveloped structure. The intact particles were released from the cell surface by a budding process in which crescent shape structures first appeared at the cell membrane, then subsequently just before release matured to a complete structure with an electron dense core. In MT/LAV-17, the synthesis of HIV-1 proteins was normal, and the particles were teardrop-shaped with an intact core structure. These particles were produced by budding with an electron dense core at the cell surface.

Thus, it was suggested that the morphological maturation of HIV-1 particles was completed just before release from the cell surface in several cell clones producing HIV-1 particles of different morphology.

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Author notes

  1. J. J. Zhang

    Present address: Division of Viral Disease, Shaanxi Anti-epidemic Station, Xian, China

Authors and Affiliations

  1. Department of Microbiology, Osaka Medical College, Takatsuki, Osaka

    T. Goto, J. J. Zhang, C. Morita, K. Sano & M. Nakai

  2. Division of Serology, Institute of Immunological Science, Hokkaido University, Kita-ku, Sapporo

    K. Ikuta

  3. Department of Pathology, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka, Japan

    S. Kato

  4. Research Center of UHVEM, Osaka University, Suita, Osaka, Japan

    M. Komatsu & H. Fujita

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Goto, T., Ikuta, K., Zhang, J.J. et al. The budding of defective human immunodeficiency virus type 1 (HIV-1) particles from cell clones persistently infected with HIV-1. Archives of Virology 111, 87–101 (1990). https://doi.org/10.1007/BF01310507

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