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Inhibition of phorbol ester-induced neutrophil chemiluminescence by FeLV

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Summary

Receptor-mediated activation is accompanied by phospholipid metabolism and by calcium fluctuation resulting in a chemiluminescence (CL) response in the neutrophil. This pathway involves activation of protein kinase C (PKC) and the NADPH oxidase. Artificial stimulants such as phorbol esters, specifically 12-O-tetradecanylphorbol-13-acetate (TPA), circumvent the receptor-mediated pathway and activate PKC resulting in a measurable CL response. Neutrophils from feline leukemia virus (FeLV) exposed cats were tested for their ability to generate a TPA-induced CL response. As compared to the non-FeLV-exposed specific-pathogen-free (SPF) control cat neutrophil CL responses, both viremic and nonviremic FeLV-exposed cats showed significant decreases in their CL responsiveness. Neither ultraviolet light-inactivated FeLV (UV-FeLV) nor protein components (FeLV-p15E and FeLV-p27) caused a significant decrease in the CL responses of the SPF cat neutrophils. The suppressed TPA-induced CL response from FeLV-infected cats may involve an intracellular mechanism not affected in vitro by exposure of the neutrophil to the virus or viral components.

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Authors and Affiliations

  1. Center for Retrovirus Research, Department of Veterinary Pathobiology, The Ohio State University, Columbus, Ohio, USA

    Charlene S. Dezzutti, L. J. Lafrado, M. G. Lewis & R. G. Olsen

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  1. Charlene S. Dezzutti
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  2. L. J. Lafrado
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  3. M. G. Lewis
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  4. R. G. Olsen
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Dezzutti, C.S., Lafrado, L.J., Lewis, M.G. et al. Inhibition of phorbol ester-induced neutrophil chemiluminescence by FeLV. Archives of Virology 111, 75–85 (1990). https://doi.org/10.1007/BF01310506

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  • DOI: https://doi.org/10.1007/BF01310506

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