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Estramustine phosphate reversibly inhibits an early stage during adenovirus replication

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Summary

Estramustine phosphate, an estradiol-mustard conjugate, was shown to reversibly inhibit a stage during the first hour of productive adenovirus 2 infection of HeLa cells. This drug, employed in the therapy of advanced prostatic cancer, specifically interacts with microtubule-associated proteins (MAPs) of the cytoskeleton. The results obtained under physiological conditions in vivo suggest a MAPs-interference with the microtubule-mediated vectorial migration of the virus inoculum to the nucleus. Virus attachment, uncoating kinetics and the appearance of established uncoating intermediates were not affected.

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Authors and Affiliations

  1. Department of Microbiology, The University of Lund, Sweden

    E. Everitt, H. Ekstrand & B. Boberg

  2. Pharmacia LEO Therapeutics, Helsingborg, Sweden

    B. Hartley-Asp

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  1. E. Everitt
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  2. H. Ekstrand
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  3. B. Boberg
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  4. B. Hartley-Asp
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Everitt, E., Ekstrand, H., Boberg, B. et al. Estramustine phosphate reversibly inhibits an early stage during adenovirus replication. Archives of Virology 111, 15–28 (1990). https://doi.org/10.1007/BF01310502

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  • DOI: https://doi.org/10.1007/BF01310502

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