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Nucleotide sequence of the coat protein coding region of the potyvirus tobacco vein-banding mosaic virus

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Summary

The sequence of the 3′ 1184 nucleotides of tobacco vein-banding mosaic virus (TVBMV) genome has been determined. It contains a single open reading frame which encompasses the whole of the coat protein of TVBMV. The sequence of the first 20 amino acids at the N-terminal region of the coat protein has also been determined chemically to be GDDQTVDAGKNVQSNQKQRN. The sequence matches the translation product of the open reading frame starting with amino acid-271; a glycine residue. Thus the coat protein of TVBMV has a calculated Mr of 30 210. The 3′ non-coding region of TVBMV is 185 nucleotides in length. Sequence alignment of the coat proteins or the 3′ non-coding regions from TVBMV and other reported potyviruses indicated that TVBMV is a separate species of the potyvirus genus.

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Authors and Affiliations

  1. Agricultural Biotechnology Laboratories, National Chung-Hsing University, Taiwan

    B. -Y. Chang, C. R. Huang & S. -D. Yeh

  2. Tobacco Research Institute, Tobacco and Wine Monopoly Bureau, Taiwan

    J. -K. Chiang, L. -M. Hung & H. -Y. Hu

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  1. B. -Y. Chang
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  2. C. R. Huang
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  3. S. -D. Yeh
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  4. J. -K. Chiang
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  5. L. -M. Hung
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  6. H. -Y. Hu
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Chang, B.Y., Huang, C.R., Yeh, S.D. et al. Nucleotide sequence of the coat protein coding region of the potyvirus tobacco vein-banding mosaic virus. Archives of Virology 138, 17–25 (1994). https://doi.org/10.1007/BF01310035

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