Summary
Reverse transcription coupled with the polymerase chain reaction (RT-PCR) was used for the rapid laboratory diagnosis of pestivirus infections. A direct DNA sequencing method was developed for the analysis of the amplified cDNA from the 5′ noncoding region of the viral genome. 70 pestivirus strains were compared in this study. Sequence analysis allowed the characterization of each isolate as either classical swine fever virus (CSFV), bovine viral diarrhea virus, or border disease virus, respectively. The 48 CSFV strains could be further classified into several subgroups, which correlated either with the geographical origin or the date of the first isolation of the respective isolate.
References
Anonymous (1993) Report from the subgroup of the scientific veterinary committee on programmes for monitoring classical swine fever. Commission of the European Communities, Directorate general for Agriculture. VI/3139/93-EN-Rev. 1 (PVET/EN/1691)
Becher P, Shannon AD, Tautz N, Thiel H-J (1994) Molecular characterization of Border disease virus, a pestivirus from sheep. Virology 198: 542–551
Collett MS (1992) Molecular genetics of pestiviruses. Comp Immun Microbiol Infect Dis 15: 145–154
Collett Ms, Larson R, Gold C, Strick D, Anderson DK, Purchio AF (1988) Molecular cloning and nucleotide sequencing of the pestivirus bovine viral diarrhea virus. Virology 165: 191–199
De Mia GM, Lowings JP, Sands JJ, Paton DJ, Rutili D (1993) Comparison of Italian swine fever isolates obtained from domestic pigs and wild boars. Report on meeting of national swine fever laboratories within the European communities, pp 37–38
Demoerlooze L, Lecomte C, Brownshimmer S, Schmetz D, Guiot C, Vandenbergh D, Allaer D, Rossius M, Chappuis G, Dina D, Renard A, Martial JA (1993) Nucleotide sequence of the bovine viral diarrhoea virus Osloss strain — comparison with related viruses and identification of specific DNA probes in the 5′ untranslated region. J Gen Virol 74: 1433–1438
Deng RT, Brock KV (1992) Molecular cloning and nucleotide sequence of a pestivirus genome, noncytopathic bovine viral diarrhea virus strain SD-1. Virology 191: 867–879
Deng RT, Brock KV (1993) 5′ and 3′ untranslated regions of pestivirus genome — primary and secondary structure analyses. Nucleic Acids Res 21: 1949–1957
Edwards S, Sands JJ, Harkness JW (1988) The application of monoclonal antibody panels to characterize pestivirus isolates from ruminants in Great Britain. Arch Virol 102: 197–206
Frölich K, Hofmann MA (1994) Isolation of bovine viral diarrhea virus (BVDV)-like pestiviruses from roe deer (Capreolus capreolus). J Wildl Dis (in press)
Hofmann MA, Brian DA (1991) Sequencing PCR DNA amplified directly from a bacterial colony. Biotechniques 11: 30–31
Katz JB, Ridpath JF, Bolin SR (1993) Presumptive diagnostic differentiation of hog cholera virus from bovine viral diarrhea and border disease viruses by using a cDNA nested-amplification approach. J Clin Microbiol 31: 565–568
Liess B, Moenning V (1990) Ruminant pestivirus infection in pigs. Rev Sci Tech Off Int Epiz 9: 151–161
Liu ST, Li SN, Wang DC, Chang SF, Chiang SC, Ho WC, Chang YS, Lai SS (1991) Rapid detection of hog cholera virus in tissues by the polymerase chain reaction. J Virol Methods 35: 227–236
Meerovitch K, Sonenberg N (1993) Internal initiation of picornavirus RNA translation. Semin Virol 4: 217–227
Meyers G, Rümenapf T, Thiel H-J (1989) Molecular cloning and nucleotide sequencing of the genome of hog cholera virus. Virology 171: 555–567
Moormann RJM, Hulst MM (1988) Hog cholera virus: Identification and characterization of the viral RNA and the virus-specific RNA synthesized in infected swine kidney cells. Virus Res 11: 281–291
Moormann RJM, Warmerdam PAM, Van der Meer B, Schaaper WMM, Wensvoort G, Hulst MM (1990) Molecular cloning and nucleotide sequence of hog cholera virus strain Brescia and mapping of the genomic region encoding envelope protein E1. Virology 177: 184–198
Paton DJ, Sands JJ, Edwards S (1994) Border disease virus: delineation by monoclonal antibodies. Arch Virol 135: 241–252
Renard A, Dino B, Martial J (1987) Vaccines and diagnostics derived from bovine diarrhea virus. Europ. Patent Application, No 86870095.6. Publication No 0208672
Ridpath JF, Bolin SR, Katz J (1993) Comparison of nucleic acid hybridization and nucleic acid amplification using conserved sequences from the 5' noncoding region for detection of bovine viral diarrhea virus. J Clin Microbiol 31: 986–989
Ruggli N, Hofmann MA, Brechtbühl K, Tratschin JD (1993) Nucleotide sequence analysis of two hog cholera virus strains (Alfort/87 and CAP). In: Proceedings of the IXth International Congress of Virology, Glasgow, Scotland, p 147
Thiel H-J, Stark R, Weiland E, Rümenapf T, Meyers G (1991) Hog cholera virus: molecular composition of virions from a pestivirus. J Virol 65: 4705–4712
Wirz B, Tratschin JD, Müller HK, Mitchell DB (1993) Detection of hog cholera virus and differentiation from other pestiviruses by polymerase chain reaction. J Clin Microbiol 31: 1148–1154
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Hofmann, M.A., Brechtbühl, K. & Stäuber, N. Rapid characterization of new pestivirus strains by direct sequencing of PCR-amplified cDNA from the 5′ noncoding region. Archives of Virology 139, 217–229 (1994). https://doi.org/10.1007/BF01309467
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DOI: https://doi.org/10.1007/BF01309467