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Biological and pharmacokinetic properties of a novel immunoglobulin-CD4 fusion protein

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Summary

We report a CD4-immunoglobulin fusion protein in which the first two extracellular domains of human CD4 replace the Fc region of the immunoglobulin. When co-expressed with a gene encoding an immunoglobulin light chain, the protein was covalently assembled into a form having an Mr consistent with that expected for two fusion heavy chains and two immunoglobulin light chains. The antigen specificity of the antibody was retained, however, binding to HIV gp120 was lost. Pharmacokinetic analysis revealed the in vivo half-life of the fusion protein to be 2.4 h in mice.

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Authors and Affiliations

  1. Department of Microbiology and Molecular Genetics and the Molecular Biology Institute, University of California, Los Angeles, California, USA

    J. B. Lyczak & S. L. Morrison

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  1. J. B. Lyczak
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  2. S. L. Morrison
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Lyczak, J.B., Morrison, S.L. Biological and pharmacokinetic properties of a novel immunoglobulin-CD4 fusion protein. Archives of Virology 139, 189–196 (1994). https://doi.org/10.1007/BF01309464

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  • DOI: https://doi.org/10.1007/BF01309464

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