Abstract
The purpose of this report is to summarize data collected over the past several years in the field of hepatocyte transplantation. Herein, a novel method of intraperitoneal transplantation of hepatocytes attached to collagen-coated microcarriers (or a totally biodegradable collagen sponge) is described, resulting in correction of specific genetic liver defects in rats and improved survival in rats undergoing 90% partial hepatectomy. Transplanted cells/matrix form well-vascularized aggregates in the peritoneal cavity; transplanted hepatocytes can be identified by light microscopy using immunohistochemical methods. Prolongation of transplanted hepatocyte survival and function was demonstrated by pretreatment of cells with ultraviolet irradiation. The ability of transplanted cells to proliferate was examined using both biochemical and morphologic criteria. Finally, a method of harvesting, cryopreserving, andin vivo functional testing of human hepatocytes is described.
This is a preview of subscription content, log in via an institution to check access.
References
Reid LM, Rojkind M: New technique for culturing differentiated cells; reconstituted basement membrane rafts. Methods Enzymol 58:263–278, 1979
Demetriou AA, Whiting J, Feldman D, Levenson SM, Chowdhury NR, Moscioni AD, Kram M, Chowdhury JR: Replacement of liver function in rats by transplantation of microcarrier-attached hepatocytes. Science 233:1190–1192, 1986
Demetriou AA, Levenson SM, Novikoff PM, Novikoff AB, Chowdhury NR, Whiting J, Reisner A, Chowdhury JR: Survival, organization and function of microcarrier-attached hepatocytes transplanted in rats. Proc Natl Acad Sci USA 83:7475–7479, 1986
Demetriou AA, Whiting J, Levenson SM, Chowdhury NR, Schechner R, Michalski S, Feldman D, Chowdhury JR: New method of hepatocyte transplantation and extracorporeal liver support. Ann Surg 204:259–271, 1986
Demetriou AA, Reisner A, Sanchez J, Levenson SM, Moscioni AD, Chowdhury JR: Transplantation of microcarrierattached hepatocytes into 90% partially hepatectomized rats. Hepatology 8:1006–1009, 1988
Barbour R, Moscioni AD, Felcher A, Demetriou AA: Survival and function of intraperitoneally transplanted hepatocytes attached to a biodegradable collagen matrix. Presented at Digestive Disease Week, Washington, DC, May 15, 1989
Felcher A, Halter S, Arepally G, Moscioni AD, nanney L, Barbour R, Demetriou AA: Immunohistochemical localization of intraperitoneally transplanted hepatocytes in analbuminemic rats. Hepatology (submitted)
Arepally G, Felcher A, Moscioni AD, Demetriou AA: Proliferation of intraperitoneally transplanted microcarrierattached rat liver cells. Surg Forum 39:396–397, 1988
Lau H, Reemtsma K, Hardy MA: Prolongation of rat islet allograft survival by direct ultraviolet irradiation of the graft. Science 233:607–609, 1984
Phay J, Felcher A, Levenson SM, Demetriou AA: Prolongation of function of transplanted microcarrier-attached hepatocytes by ultraviolet irradiation. Surg Forum 39:398–399, 1988
Moscioni AD, Chowdhury JR, Barbour R, Brown L, Chowdhury NR, Competiello L, Lahiri P, Demetriou AA: Human liver cell transplantation. Gastroenterology 96:1546–1551, 1989
Author information
Authors and Affiliations
Additional information
Supported by National Institutes of Health (NIDDK) grant DK38763 and a Veterans Administration Merit Review Award.
Rights and permissions
About this article
Cite this article
Demetriou, A.A., Felcher, A. & Moscioni, A.D. Hepatocyte transplantation. Digest Dis Sci 36, 1320–1326 (1991). https://doi.org/10.1007/BF01307530
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01307530