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Mucosal humoral immune response toHelicobacter pylori in patients with duodenitis

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Abstract

The humoral immune response toHelicobacter pylori infection in the duodenum has been investigated by short-termin vitro culture, ELISA, and immunoblotting techniques.H. pylori IgA secretion by duodenal bulb biopsies was significantly increased (P<0.001) in patients with duodenitis. The IgA response toH. pylori in patients with duodenitis was restricted to the first part of the duodenum; second part duodenal biopsies secreting significantly (P<0.001) less IgA during culturein vitro. H. pylori IgG antibody secretion by cultured biopsies was also significantly increased (P<0.01) in patients with duodenitis and those with gastricH. pylori infection but without duodenitis. Immunoblotting of duodenal bulb culture supernatants showed positive recognition by the mucosal IgA response ofH. pylori antigens in the region of 120, 90, 61, and 31–26 kDa in patients with duodenitis. Serologically, such patients showed little evidence of IgAH. pylori antibodies by immunoblotting. These results demonstrate that the inflammatory response in the duodenal mucosa of patients with duodenitis represents a specific highly localized humoral response toH. pylori.

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Authors and Affiliations

  1. Departments of Medicine and Pathology, St. James's University Hospital, LS9 7TF, Leeds, UK

    J. E. Crabtree D. Phil, T. M. Shallcross MB, BS, J. I. Wyatt MB BS, J. D. Taylor PhD, R. V. Heatley MD, B. J. Rathbone MD & M. S. Losowsky MD

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  1. J. E. Crabtree D. Phil
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  2. T. M. Shallcross MB, BS
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  3. J. I. Wyatt MB BS
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  4. J. D. Taylor PhD
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  5. R. V. Heatley MD
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  6. B. J. Rathbone MD
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  7. M. S. Losowsky MD
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Additional information

This study was undertaken with financial support from the Yorkshire Regional Health Authority, the Medical Research Council, and Glaxo plc.

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Crabtree, J.E., Shallcross, T.M., Wyatt, J.I. et al. Mucosal humoral immune response toHelicobacter pylori in patients with duodenitis. Digest Dis Sci 36, 1266–1273 (1991). https://doi.org/10.1007/BF01307520

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  • DOI: https://doi.org/10.1007/BF01307520

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