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Regulatory peptides in lower esophageal sphincter of pig and man

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Abstract

Smooth muscle specimens from the lower esophageal sphincter (LES) region of pig and man were analyzed for vasoactive intestinal peptides (VIP), substance P (SP), enkephalin, neuropeptide Y (NPY), gastrin/cholecystokinin (CCK), neurotensin, and somatostatin using immunocytochemistry and radioimmunoassay. VIP-, SP-, enkephalin-, and NPY-immunoreactive nerve fibers were observed in the LES of both species, whereas nerve The peptide-containing nerve fibers occurred in the intramural ganglia and in the smooth muscle layers. There was a rich supply of VIP-and NPY-immunoreactive fibers, whereas the supply of SP-and enkephalin-immunoreactive nerve fibers were moderate in number in both species examined. The concentration of VIP, SP, enkephalin and NPY was comparable in the two species. The present study shows that the pattern of peptidergic innervation of the LES is similar in pig and man. It is proposed that neuronal VIP, SP, enkephalin, and NPY may serve to modulate the motor activity of the LES and that the pig is a suitable experimental animal for the study of regulatory peptides and LES functions.

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Authors and Affiliations

  1. Department of Thoracic Surgery R, University of Copenhagen, Blegdamsvej 9, DK-2100, Copenhagen Ø, Denmark

    Søren Aggestrup, Rolf Uddman, Steen Lindkær Jensen, Rolf Håkanson, Frank Sundler, Ove Schaffalitzky De Muckadell & Piers Emson

  2. Department of Internal Medicine M, Rigshospitalet, University of Copenhagen, Denmark

    Søren Aggestrup, Rolf Uddman, Steen Lindkær Jensen, Rolf Håkanson, Frank Sundler, Ove Schaffalitzky De Muckadell & Piers Emson

  3. Department of Otolaryngology, Malmö General Hospital, Sweden

    Søren Aggestrup, Rolf Uddman, Steen Lindkær Jensen, Rolf Håkanson, Frank Sundler, Ove Schaffalitzky De Muckadell & Piers Emson

  4. Departments of Histology and Pharmacology, University of Lund, Sweden

    Søren Aggestrup, Rolf Uddman, Steen Lindkær Jensen, Rolf Håkanson, Frank Sundler, Ove Schaffalitzky De Muckadell & Piers Emson

  5. MRC Neurochemical Pharmacology, Unit, Medical School, Cambridge, England

    Søren Aggestrup, Rolf Uddman, Steen Lindkær Jensen, Rolf Håkanson, Frank Sundler, Ove Schaffalitzky De Muckadell & Piers Emson

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  1. Søren Aggestrup
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  2. Rolf Uddman
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  3. Steen Lindkær Jensen
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  4. Rolf Håkanson
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  5. Frank Sundler
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  6. Ove Schaffalitzky De Muckadell
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  7. Piers Emson
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Aggestrup, S., Uddman, R., Jensen, S.L. et al. Regulatory peptides in lower esophageal sphincter of pig and man. Digest Dis Sci 31, 1370–1375 (1986). https://doi.org/10.1007/BF01299816

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  • DOI: https://doi.org/10.1007/BF01299816

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