Summary
The ultrastructure of the flagellar apparatus of aPleurochrysis, a coccolithophorid was studied in detail. Three major fibrous connecting bands and several accessory fibrous bands link the basal bodies, haptonema and microtubular flagellar roots. The asymmetrical flagellar root system is composed of three different microtubular roots (referred to here as roots 1,2, and 3) and a fibrous root. Root 1, associated with one of the basal bodies, is of the compound type, constructed of two sets of microtubules,viz. a broad sheet consisting of up to twenty closely aligned microtubules, and a secondary bundle made up of 100–200 microtubules which arises at right angles to the former. A thin electron-dense plate occurs on the surface of the microtubular sheet opposite the secondary bundle. The fibrous root arises from the same basal body and passes along the plasmalemma together with the microtubular sheet of root 1. Root 2 is also of the compound type and arises from one of the major connecting bands (called a distal band) as a four-stranded microtubular root and extends in the opposite direction to the haptonema. From this stranded root a secondary bundle of microtubules arises at approximately right angle. Root 3 is a more simple type, composed of at least six microtubules which are associated with the basal body. The flagellar transition region was found to be unusual for the classPrymnesiophyceae. The phylogenetic significance of the flagellar apparatus in thePrymnesiophyceae is discussed.
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References
Chretienot, M., 1973: The fine structure and taxonomy ofPlatychrysis pigra Geitler (Haptophyceae). J. mar. biol. Ass. U.K.53, 905–914.
Gayral, P., Fresnel, J., 1976: Nouvelles observations sur deuxCoccolithophoracées marines:Cricosphaera roscoffensis (P. Dangeard) comb. nov. etHymenomonas globosa (F. Magne) comb. nov. Phycologia15, 339–355.
— —, 1983: Description, sexualite et cycle de developpement d'une nouvelleCoccolithophoracée (Prymnesiophyceae): Pleurochrysis pseudoroscoffensis sp. nov. Protistologica19, 245–261.
-Lepailleur, H., 1972: Alternance morphologique de generations et alternance de phases chez les Chrysophycees. Soc. bot. Fr. Mém. 215–230.
Green, J. C., 1973: Studies in the fine structure and taxonomy of flagellates in the genusPavlova. II. A freshwater representative,Pavlova granifera (Mack) comb. nov. Brit. phycol. J.8, 1–12.
—, 1976: Notes on the flagellar apparatus and taxonomy ofPavlova mesolychnon van der Veer, and on the status ofPavlova Butcher and related genera within theHaptophyceae. J. mar. biol. Ass. U.K.56, 595–602.
—, 1980: The fine structure ofPavlova pinguis Green and a preliminary survey of the orderPavlovales (Prymnesiophyceae). Brit. phycol. J.15, 151–191.
—,Hibberd, D. J., 1977: The ultrastructure and taxonomy ofDiacronema vlkianum (Prymnesiophyceae) with special reference to the haptonema and flagellar apparatus. J. mar. biol. Ass. U.K.57, 1125–1136.
—,Pienaar, R. N., 1976: The taxonomy of the orderIsochrysidales (Prymnesiophyceae) with special reference to the generaIsochrysis Parke,Diacronema Parke andImantonia Reynolds. J. mar. biol. Ass. U.K.57, 7–17.
Hibberd, D. J., 1979: The structure and phylogenetic significance of the flagellar transition region in the chlorophyll c containing algae. BioSystems11, 243–261.
—, 1980:Prymnesiophytes (= Haptophytes). In: Phytoflagellates. Developments in marine biology, Vol. 2 (Cox, E. R., ed.), pp. 273–317. North-Holland: Elsevier.
Inouye, I., Chihara, M., 1979: Life history and taxonomy ofCricosphaera roscoffensis var.haptonemofera var. nov. (classPrymnesiophyceae) from the Pacific. Bot. Mag. Tokyo92, 75–87.
— —, 1980: Laboratory culture and taxonomy ofHymenomonas coronata andOchrosphaera verrucosa (classPrymnesiophyceae) from the Northwest Pacific. Bot. Mag. Tokyo93, 195–208.
— —, 1983: Ultrastructure and taxonomy ofJomonlithus littoralis gen. et sp. nov. (classPrymnesiophyceae), a coccolithophorid from the Northwest Pacific. Bot. Mag. Tokyo96, 365–376.
-Pienaar, R. N., 1984: New observations on the coccolithophoridUmbilicosphaera sibogae var.foliosa (Prymnesiophyceae) with reference to cell covering, cell structure and flagellar apparatus. Brit. phycol. J. (in press).
Klaveness, D., 1973: The microanatomy ofCalyptrosphaera sphaeroidea, with some supplementary observations on the motile stage ofCoccolithus pelagicus. Norw. J. Bot.20, 151–162.
Leadbeater, B. S. C., 1971: Observations on the life history of the haptophycean algaPleurochrysis scherffelii with special reference to the microanatomy of the different types of motile cells. Ann. Bot.35, 429–439.
Manton, I., 1964 a: The possible significance of some details of flagellar bases in plants. J. R. Microsc. Sc.82, 279–285.
—, 1964 b: Further observations on the fine structure of the haptonema inPrymnesium parvum. Arch. Mikrobiol.49, 315–330.
—, 1966: Observations on scale production inPrymnesium parvum. J. Cell Sci.1, 375–380.
—, 1968: Further observations on the microanatomy of the haptonema inChrysochromulina chiton andPrymnesium parvum. Protoplasma66, 35–53.
—,Peterfi, L. S., 1969: Observations on the fine structure of coccoliths, scales and the protoplast of a freshwater coccolithophorid,Hymenomonas roseola Stein, with supplementary observations on the protoplast ofCricosphaera carterae. Proc. Roy. Soc. B.175, 1–15.
Melkonian, M., 1982: Structural and evolutionary aspects of the flagellar apparatus in green algae and land plants. Taxon31, 255–265.
Mills, J. T., 1975:Hymenomonas coronata sp. nov., a new coccolithophorid from the Texas coast. J. Phycol.11, 149–154.
Moestrup, Ø., 1978: On the phylogenetic validity of the flagellar apparatus in green algae and other chlorophyll a and b containing plants. BioSystems10, 117–144.
—, 1982: Flagellar structure in algae: a review, with new observations particularly on theChrysophyceae, Phaeophyceae (Fucophyceae), Euglenophyceae, andReckertia. Phycologia21, 427–528.
Parke, M.,Green, J. C., 1976:Haptophyta. In: Check-lists of British marine algae—Third revision. (Parke, M.,Dixon, P., eds.), J. mar. biol. Ass. U.K.56, 527–594.
— —,Manton, I., 1971: Observations on the fine structure of zoids of the genusPhaeocystis (Haptophyceae). J. mar. biol. Ass. U.K.51, 927–941.
Pienaar, R. N., 1976: The microanatomy ofHymenomonas lacuna sp. nov. (Haptophyceae). J. mar. biol. Ass. U.K.56, 1–11.
Provasoli, L., 1968: Media and prospects for cultivation of marine algae. In: Culture and Collection of Algae. Proc. U.S.-Japan Conf., Hakone, Sept. 1966 (Watanabe, A.,Hattori, A., eds.), pp. 63–85. Jap. Soc. Plant Physiol.
Reynolds, E. S., 1963: The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J. Cell Biol.17, 208–212.
Stewart, K., Mattox, K., 1978: Structural evolution in the flagellated cells of green algae and land plants. BioSystems10, 145–152.
Von Stosch, H. A., 1967:Haptophyceae. In: Vegetative Fortpflanzung, Parthenogenese und Apogamie bei Algen. Encyclopedia of plant physiology18 (Ruhland, W., ed.), pp. 646–656. Berlin-Heidelberg-New York: Springer.
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Inouye, I., Pienaar, R.N. Ultrastructure of the flagellar apparatus inPleurochrysis (classPrymnesiophyceae). Protoplasma 125, 24–35 (1985). https://doi.org/10.1007/BF01297347
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DOI: https://doi.org/10.1007/BF01297347