Abstract
In rat colon damaged by 10% acetic acid and by dinitrochlorobenzene, we test the following hypotheses: (1) mucosal hemodynamic changes are significantly different at the ulcer base, the ulcer margin, and the inflamed non-ulcer-bearing mucosa; and (2) these mucosal hemodynamic changes also vary with time after induction of the colonic injury. Mucosal hemodynamic changes were documented by reflectance spectrophotometry, and variations in gross mucosal morphology were confirmed by hematoxylin and eosin histologic sections. Results revealed that in the acute stage, the ulcer base, which was covered by necrotic debris, showed ischemia without congestion. The ulcer margin at the edge of the ulcer base showed ischemia with congestion. The nonulcerated mucosa, which appeared erythematous, showed increased perfusion. In the convalescent stage, all the altered perfusion patterns returned to normal. These observations offer plausible explanations for the variability in colonic perfusion observed in experimentally damaged colons.
Similar content being viewed by others
References
Brown JA, Zipser RD: Prostaglandin regulation of colonic blood flow in rabbit colitis. Gastroenterology 2:54–59, 1987
Sekizuka E, Grisham MB, Li M, Deitch E, Granger DN: Inflammation-induced intestinal hyperemia in the rat: Role of neutrophils. Gastroenterology 95:1528–1534, 1988
Leung FW, Koo A: Mucosal vascular stasis precedes loss of viability of endothelial cells in rat acetic acid colitis. Dig Dis Sci 36:727–732, 1991
Leung FW: Role of capsaicin-sensitive afferent nerves in mucosal injury and injury-induced hyperemia in rat colon. Am J Physiol 262:G332-G337, 1992
MacPherson BR, Pfeiffer CJ: Experimental production of diffuse colitis in rats. Digestion 17:135, 1978
Rabin BS, Rogers SJ: A cell-mediated immune model of inflammatory bowel disease in the rabbit. Gastroenterology 75:29–33, 1978
Leung FW, Morishita T, Livingston EH, Reedy T, Guth PH: Reflectance spectrophotometry for the assessment of gastroduodenal mucosal perfusion. Am J Physiol 252:G797-G804, 1987
Leung FW, Slodownik E, Jensen DM, Van Deventer GM, Guth PH: Gastroduodenal mucosal hemodynamics by endoscopic reflectance spectrophotometry. Gastrointest Endosc 33:284–288, 1987
Leung FW, Guth PH, Scremin O, Golanska E, Kauffman G Jr. Regional gastric mucosal blood flow measurements by hydrogen gas clearance in the anesthetized rat and rabbit. Gastroenterology 87:28–36, 1984
Endoh K, Kauffman GL Jr, Leung FW: Mechanism of aggravation of mucosal injury by intravenous nicotine in rat stomach. Am J Physiol 261:G1037-G1042, 1991
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Leung, F.W., Su, K.C., Yonel, Y. et al. Regional differences in mucosal hemodynamics in experimental colonic injury in rats. Digest Dis Sci 38, 1220–1223 (1993). https://doi.org/10.1007/BF01296070
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01296070