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Cytoplasmic vesicles in germinating spores ofGilbertella persicaria

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Summary

Germ tube apices ofGilbertella persicaria contain cytoplasmic vesicles, similar to the secretory vesicles found at the tips of vegetative hyphae. The vesicles are present at all stages of development, from the time of germ tube initiation to the establishment of branched hyphae. In contrast to the abundant vesicles at tips of established hyphae, the germ tubes have only a few apical vesicles in a layer next to the plasma membrane. When germinated spores are treated by washing and centrifuging prior to fixation, the cytoplasm is often disrupted near the apex, and the clusters of apical vesicles disappear. The findings indicate the delicate nature of hyphal tips and the necessity of avoiding prefixation stresses in order to preserve the apical apparatus of growing hyphae.

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  1. Charles E. Bracker

    Present address: Department of Botany and Plant Pathology, Purdue University, Lafayette, Indiana, USA

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    1. Charles E. Bracker
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    Bracker, C.E. Cytoplasmic vesicles in germinating spores ofGilbertella persicaria . Protoplasma 72, 381–397 (1971). https://doi.org/10.1007/BF01289510

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