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Direct interaction between glyoxysomes and lipid bodies in cotyledons of theArabidopsis thaliana ped1 mutant

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Summary

During germination and subsequent growth of fatty seeds, higher plants obtain energy from the glyconeogenic pathway in which fatty acids are converted to succinate in glyoxysomes, which contain enzymes for fatty acid β-oxidation and the glyoxylate cycle. TheArabidopsis thaliana ped1 gene encodes a 3-ketoacyl-CoA thiolase (EC 2.3.1.16) involved in fatty acid β-oxidation. Theped1 mutant shows normal germination and seedling growth under white light. However, etiolated cotyledons of theped1 mutant grow poorly in the dark and have small cotyledons. To elucidate the mechanisms of lipid degradation during germination in theped1 mutant, we examined the morphology of theped1 mutant. The glyoxysomes in etiolated cotyledons of theped1 mutant appeared abnormal, having tubular structures that contained many vesicles. Electron microscopic analysis revealed that the tubular structures in glyoxysomes are derived from invagination of the glyoxysomal membrane. By immunoelectron microscopic analysis, acyl-CoA synthetase (EC 6.2.1.3), which was located on the membrane of glyoxysomes in wild-type plants, was located on the membranes of the tubular structures in the glyoxysomes in theped1 mutant. These invagination sites were always in contact with lipid bodies. The tubular structure had many vesicles containing substances with the same electron density as those in the lipid bodies. From these results, we propose a model in which there is a direct mechanism of transporting lipids from the lipid bodies to glyoxysomes during fatty acid β-oxidation.

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Authors and Affiliations

  1. Department of Cell Biology, National Institute for Basic Biology, 444-8585, Okazaki, Japan

    Y. Hayashi, M. Hayashi, H. Hayashi & M. Nishimura

  2. Department of Environmental Science, Faculty of Science, Niigata University, Niigata

    Y. Hayashi

  3. Department of Botany, Graduate School of Science, Kyoto University, Kyoto

    I. Hara-Nishimura

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  1. Y. Hayashi
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  4. I. Hara-Nishimura
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  5. M. Nishimura
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Hayashi, Y., Hayashi, M., Hayashi, H. et al. Direct interaction between glyoxysomes and lipid bodies in cotyledons of theArabidopsis thaliana ped1 mutant. Protoplasma 218, 83–94 (2001). https://doi.org/10.1007/BF01288364

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