Summary
Electron micrographs ofSaccharomycopsis lipolytica-cells grown in a medium with lactate as carbon source and fixed with glutaraldehyde-OsO4 or KmnO4 show 3–4 profiles of microbodies per section in average. The number of microbodies is about three times greater when cells are cultivated in a medium with n-hexadecane. The spherical to avoid microbodies (0.3–0,8 μm in diameter) have a homogeneous matrix.
After conversion of cells into protoplasts by the aid of snail gut juice tubular inclusions occur in microbodies. The single tubulus has an outer diameter of about 25 nm, a length up to 0.8 μm and contains a central rod. Several tubules form bundles or layers. It is suggested that the tubules could be enzyme protein assembling by osmotic shock with hypertonic solutions during preparation of protoplasts.
Zusammenfassung
Auf Ultradünnschnitten Glutaraldehyd-OsO4 oder KMnO4 fixierterSaccharomycopsis lipolytica-Zellen, die in einem Medium mit Laktat als C-Quelle wuchsen, werden durchschnittlich 3–4 Anschnitte von Microbodies gefunden. Ihre Anzahl erhöht sich etwa um das Dreifache, wenn n-Hexadecan die C-Quelle ist.
Nach der Umwandlung der Zellen zu Protoplasten mit Hilfe von Enzymgemischen vonHelix pomatia enthalten die Microbodies tubuläre Einschlüsse. Diese Tubuli, deren äußerer Durchmesser etwa 25 nm beträgt, haben eine zentrale Verdichtung und erreichen Längen bis zu 0,8 μm. Sie sind in Bündeln oder Schichten angeordnet. Die Tubuli bilden sich möglicherweise durch Assemblierung von Enzymproteinen, wenn die Zellen während der Umwandlung zu Protoplasten mit hypertonischen Lösungen osmotisch geschockt werden.
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May, R., Barth, G. Tubuläre Einschlüsse in Microbodies vonSaccharomycopsis (Candida) lipolytica-Protoplasten. Protoplasma 91, 83–91 (1977). https://doi.org/10.1007/BF01284197
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DOI: https://doi.org/10.1007/BF01284197