Summary
The effect of caffeine on cell plate formation inTradescantia stamen hair cells has been studiedin vivo using Nomarski differential interference contrast microscopy. It is well known that caffeine is a potent inhibitor of cell plate formation. Direct examination of drug-treated cells reveals that the cell plate always arises and grows centrifugally until almost complete. Up to this point drug-treated cells are indistinguishable from controls. However in the presence of caffeine the plate never reaches completion but rather appears to melt away until no refractile structure remains. Even after cells have been cultured for 24 hours in caffeine a cell plate always arises only to subsequently break down. Studies on the time of caffeine action show that within minutes before the onset of cell plate formation, the drug efficiently reaches the target site. This effect is partially reversible by washing out the caffeine with HEPES/KCl buffer, however the time required for the cell plate to reach completion is prolonged and in some instances the plate appears to be fragmented. Adenosine is also partially effective in reversing the caffeine inhibition of cell plate formation.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Amrhein, N., 1974: Evidence against the occurrence of adenosine 3′5′ cAMP in higher plants. Planta118, 241–258.
Becerra, J., López-Sáez, J. F., 1978: Effects of caffeine, calcium and magnesium on plant cytokinesis. Exp. Cell Res.111, 301–308.
Bianchi, C. P., 1968: Pharmacological action on excitationcontraction coupling in striated muscle. Fed. Proc.27, 126–131.
Brown, E. G., Newton, R. P., 1981: Cyclic AMP and higher plants. Phytochemistry20, 2453–2463.
Butcher, R. W., Sutherland, E. W., 1962: Adenosine-3′5′-phosphate in biological materials. 1 purification and properties of 3′5′-nucleotide phosphodiesterase and use of this enzyme to charactorize adenosine 3′5′ phosphate in human urine. J. biol. Chem.237, 1244–1250.
Deysson, G., Benbadis, M. C., 1966: Modalités de l'action inhibitrice de la caféine sur la cytodiérèse dans les cellules méristématiques radiculaires d'Allium sativum. J. Microsc.5, 511–518.
Dietrich, J., 1975: Etude ultrastructurale du mode d'action de la caféine dans l'inhibition de la cytodiérèse de cellule végétale. Le Botaniste57, 75–84.
Endo, M., 1977: Calcium release from sarcoplasmic reticulum. Physiol. Rev.57, 71–108.
Fain, J., Malbon, C., 1979: Regulation of adenylate cyclase by adenosine. Molec. and cell. Biochem.25, 143–169.
González-Fernández, A., López-Sáez, J. F., 1980: The effect of adenosine on caffeine inhibition of plant cytokinesis. Environ. and exp. Bot.20, 455–461.
Gunning, B. E. S., 1982: The cytokinetic apparatus: its development and spacial regulation. In: The cytoskeleton in plant growth and development (Lloyd, C. W., ed.), pp. 230–288. New York: Academic Press.
Hepler, P. K., 1980: Membranes in the mitotic apparatus of barley cells. J. Cell Biol.86, 490–499.
—, 1985: Calcium and plant development. Ann. Rev. Plant Physiol.,36, 397–439.
Jones, M. G., Payne, H. L., 1978: Cytokinesis inImpatiens balsamina and the effect of caffeine. Cytobios20, 79–91.
Jost, J. P., Rickenberg, H. V., 1971: Cyclic AMP. Ann. Rev. Biochem.40, 741–774.
López-Sáez, J. F., Risueño, M. C., Giménez-Martín, G., 1966: Inhibition of cytokinesis in plant cells. J. Ultrastruct. Res.14, 85–94.
Paul, D. C., Goff, C. W., 1973: Comparative effects of caffeine, its analogues and calcium deficiency on cytokinesis. Exp. Cell Res.78, 399–413.
Pickett-Heaps, J. D., 1969: Preprophase microtubule bands in some abnormal mitotic cells of wheat. J. Cell Sci.4, 397–420.
Poste, G., Allison, A. C., 1971: Membrane fusion reaction: a theory. J. theor. Biol.32, 165–184.
Rasmussen, H., Tenenhouse, A., 1968: Cyclic adenosine monophosphate, Ca++, and membranes. Biochemistry59, 1364–1370.
Risueño, M. C., Giménez-Martín, G., López-Sáez, J. F., 1968: Experimental analysis of plant cytokinesis. Exp. Cell Res.49, 136–147.
Robison, G. A., Butcher, R. W., Sutherland, E. W., 1968: Cyclic AMP. Ann. Rev. Biochem.37, 149–174.
Satin, A., Rall, T. W., 1970: The effects of adenosine and adenine nucleotides on the cyclic adenosine phosphate content of guinea pig cerebral cortex slices. Molec. Pharm.6, 13–23.
Weber, A., 1968: The mechanism of the action of caffeine on sarcoplasmic reticulum. J. gen. Physiol.52, 760–772.
—, 1968: The relationship between caffeine contraction of intact muscle and the effect of caffeine on reticulum. J. gen. Physiol.52, 750–759.
Yamamoto, N., Kasai, M., 1982: Kinetics of the action of caffeine and procaine on the Ca2+ gated cation channel in sarcoplasmic reticulum vesicles. J. Biochem.92, 477–484.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Bonsignore, C.L., Hepler, P.K. Caffeine inhibition of cytokinesis: Dynamics of cell plate formation-deformationin vivo . Protoplasma 129, 28–35 (1985). https://doi.org/10.1007/BF01282302
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01282302