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Rearrangement of the actin filament and microtubule cytoskeleton during induction of microspore embryogenesis inBrassica napus L. cv. Topas

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Summary

Changes in the actin filament and microtubule cytoskeleton were examined during heat- and cytochalasin D-induced embryogenesis in microspores ofBrassica napus cv. Topas by rhodamine phalloidin and immunofluorescence labelling respectively. The nucleus was displaced from its peripheral to a more central position in the cell, and perinuclear actin microfilaments and microtubules extended onto the cytoplasm. Heat treatment induced the formation of a preprophase band of microtubules in microspores; preprophase bands are not associated with the first pollen mitosis. Actin filament association with the preprophase band was not observed. The orientation and position of the mitotic spindle were altered, and it was surrounded with randomly oriented microfilaments. The phragmoplast contained microfilaments and microtubules, as in pollen mitosis I, but it assumed a more central position. Cytoskeletal reorganisation also occurred in microspores subjected to a short cytochalasin D treatment, in the absence of a heat treatment. Cytochalasin D treatment of microspores resulted in dislocated mitotic spindles, disrupted phragmoplasts, and symmetric divisions and led to embryogenesis, confirming that a normal actin cytoskeleton has a role in preventing the induction of embryogenesis.

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Abbreviations

CD:

cytochalasin D

MF:

actin microfilament

MT:

microtubule

PPB:

preprophase band

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Authors and Affiliations

  1. Eastern Cereals and Oilseeds Research Centre, Agriculture and Agri-Food Canada, Central Experimental Farm Bldg 21, K1A 0C6, Ottawa, Ontario, Canada

    C. Gervais & D. H. Simmonds

  2. Department of Biology, Queen's University, Kingston, Ontario

    C. Gervais & W. Newcomb

Authors
  1. C. Gervais
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  2. W. Newcomb
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  3. D. H. Simmonds
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Gervais, C., Newcomb, W. & Simmonds, D.H. Rearrangement of the actin filament and microtubule cytoskeleton during induction of microspore embryogenesis inBrassica napus L. cv. Topas. Protoplasma 213, 194–202 (2000). https://doi.org/10.1007/BF01282157

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  • DOI: https://doi.org/10.1007/BF01282157

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