Summary
Flax hypocotyl protoplasts were embedded in agarose and alginate beads. The pectin molecules of the formed colonies were observed in electron microscopy using 2F4 antibody specific of a calcium-induced supramolecular conformation of homopolygalacturonic acid. Little pectin, mostly methylesterified, was present in agarose-entrapped colonies. The regenerating cells immobilized in alginate secreted much higher amounts of methylesterified pectins in their walls. De-esterification of the pectins was clearly seen after 6 days of culture. These results illustrate the importance of the external matrix on wall differentiation.
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Abbreviations
- BSA:
-
bovine serum albumine
- HV:
-
high viscosity
- MV:
-
medium viscosity
- MoAB:
-
monoclonal antibody
- PME:
-
pectin methylesterase
- PAR:
-
photosynthetically active radiation
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David, H., Bade, P., David, A. et al. Pectins in walls of protoplast-derived cells imbedded in agarose and alginate beads. Protoplasma 186, 122–130 (1995). https://doi.org/10.1007/BF01281322
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DOI: https://doi.org/10.1007/BF01281322