Summary
The purity of the chloroplasts isolated from the algaCodium fragile by 1. a conventional centrifugation method, and 2. a method involving a gel filtration stage through Sephadex G 50 coarse, was compared using enzymatic analysis in conjunction with electron microscopy. The findings show that the purer chloroplasts, obtained by gel filtration, could carboxylate more14CO2 and evolve more O2 than the crude preparation from which they originated.
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Cobb, A.H. The relationship of purity to photosynthetic activity in preparations ofCodium fragile chloroplasts. Protoplasma 92, 137–146 (1977). https://doi.org/10.1007/BF01280206
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DOI: https://doi.org/10.1007/BF01280206