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Food capture and adhesion by the heliozoonActinophrys sol

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Summary

The heliozoonActinophrys sol is characterized by needle-like axopodia radiating from the spherical cell body. When helio-zoons capture food organisms, the prey is caught by adhesion to the surface of axopodia where numerous extrusomes are present close to the plasma membrane. To understand the molecular mechanism by which the heliozoons capture prey organisms, crude isolation and characterization of the adhesive substance was carried out. Prey flagellates (Chlorogonium elongatum) adhered and aggregated to remnants of heliozoon cells which had been killed by freezing or treatment at high temperature (80 °C for 10 min). Isolated extrusomes, which were prepared as the supernatant of cells homogenized and centrifuged after freezing and thawing, showed strong adhesion to the prey flagellates which responded to the supernatant by adhering their flagella and cell bodies to each other to form bouquet-like cell clusters. The adhesive substance was further extracted from heat-treatedA. sol. This fraction contained filamentous material similar to the secreted contents of the extrusomes observed during feeding. Its adhesive activity was not inhibited by trypsin treatment.

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Authors and Affiliations

  1. Laboratory of Protistology, Department of Biology, Faculty of Science, Kobe University, 1-1 Rokkodai-cho, Nada-ku, 657-8501, Kobe, Japan

    M. Sakaguchi & T. Suzaki

  2. Institute of Zoology, Free University of Berlin, Berlin

    M. Sakaguchi & K. Hausmann

Authors
  1. M. Sakaguchi
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  2. K. Hausmann
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  3. T. Suzaki
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Correspondence to T. Suzaki.

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Sakaguchi, M., Hausmann, K. & Suzaki, T. Food capture and adhesion by the heliozoonActinophrys sol . Protoplasma 203, 130–137 (1998). https://doi.org/10.1007/BF01279469

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  • DOI: https://doi.org/10.1007/BF01279469

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