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Ultrarapid endocytotic uptake of large molecules inDunaliella species

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Summary

This paper describes the uptake of Lucifer Yellow carbohydrazide and fluorescent dextrans labeled with fluorescein isothiocyanate or Sodium Green (molecular masses ranging from 522 to 2 × 106 Da) byDunaliella spp. halotolerant unicellular green algae isolated from salt pools in the Sinai peninsula. The fluorescent dyes were taken up into a set of vesicles around the nucleus and just above the chloroplast. It proved impossible to inhibit uptake of the fluorescent compounds in cells treated with a large variety of metabolic and other inhibitors. Cell labeling was complete within half a minute of addition of fluorescent compounds to the outside medium; efflux was equally rapid. The results are interpreted in terms of an endocytotic process whereby the outside medium, together with any substance dissolved in it, remains within vesicles enclosed within the cell body but cycles rapidly between the plasma membrane and the interior of the cell. The outside medium does not pass across the vesicular membrane, nor enters the cytosol.

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Abbreviations

LYCH:

Lucifer Yellow carbohydrazide

FITC:

fluorescein-5-isothiocyanate

TCA:

trichloroacetic acid

DMSO:

dimethylsulfoxide

NEM:

N-ethyl maleimide

DNP:

dinitrophenol

CCCP:

m-chlorocarbonyl-cyanide phenylhydrazone

APM:

amiprophos-methyl

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Authors and Affiliations

  1. Section of Plant Biology, Cornell University, Ithaca, New York

    M. Ginzburg, B. Z. Ginzburg & R. Wayne

  2. Plant Biophysical Laboratory, Botany Department, Institute of Life Sciences, The Hebrew University of Jerusalem, Givat Ram, 91904, Jerusalem, Israel

    M. Ginzburg & B. Z. Ginzburg

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  1. M. Ginzburg
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  2. B. Z. Ginzburg
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  3. R. Wayne
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Ginzburg, M., Ginzburg, B.Z. & Wayne, R. Ultrarapid endocytotic uptake of large molecules inDunaliella species. Protoplasma 206, 73–86 (1999). https://doi.org/10.1007/BF01279254

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  • DOI: https://doi.org/10.1007/BF01279254

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