Summary
In cells of developing pea cotyledons fixed at ambient temperature, electron-dense cisternae often encircle vacuoles and occasionally are continuous with vacuolar protein deposits. In cotyledons chilled prior to fixation, the cisternae are present as an anastomosing network of channels that make multiple contacts with vacuolar protein. It is suggested that during fixation at ambient temperature, many of the contacts with vacuoles are lost and that much of the complexity of the network is lost, giving way to a system of largely unbranched cisternae. The network may be a mobile phase involved in reserve protein accumulation and concentration within the vacuole.
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Craig, S. Fixation of a vacuole-associated network of channels in protein-storing pea cotyledon cells. Protoplasma 135, 67–70 (1986). https://doi.org/10.1007/BF01276999
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DOI: https://doi.org/10.1007/BF01276999