Summary
Plasma membranes isolated from onion roots by twophase partition contain at least two different NAD(P)H-dehydrogenases. A 27 kDa electron transport protein oxidises both NADH and NADPH and exhibits maximal activity with quinones as electron acceptors. A distinct 31 kDa dehydrogenase is specific for NADH as donor and shows maximal activity with ferricyanide. This novel enzyme is responsible for most NADH-ferricyanide oxidoreductase activity of solubilized onion root plasma membranes and exhibits properties different to other purified NAD(P)H-dehydrogenases.
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Abbreviations
- DES:
-
diethylstilbestrol
- FeCN:
-
potassium ferricyanide
- NBT:
-
nitroblue tetrazolium
- PHMB:
-
p-hydroxymercuribenzoate
- PMSF:
-
phenylmethylsulfonylfluoride
- PTA:
-
phosphotungstic acid
- SHAM:
-
salicylhydroxamic acid
- TTFA:
-
thenoyltrifluoroacetone
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Serrano, A., Córdoba, F., González-Reyes, J.A. et al. NADH-specific dehydrogenase from onion root plasma membrane: purification and characterization. Protoplasma 184, 133–139 (1995). https://doi.org/10.1007/BF01276910
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DOI: https://doi.org/10.1007/BF01276910