Summary
Ascorbate and its free radical potentiates proliferation of HL-60 cells in serum-limiting media. Dehydroascorbate does not affect growth. This stimulation of growth is due to a general shortening of the cell cycle. The incubation of HL-60 cells with ascorbate free radical produces a significant change of the redox potential of cells. The presence of cells in culture media avoids the total oxidation of ascorbate, and also HL-60 cells induce the short-term stabilization of ascorbate. Ascorbate free radical potentiates also the onset of DNA synthesis in CCL39 cells induced by fetal calf serum, although itself does not affect quiescense to proliferation transition. This transition induced by fetal calf serum also potentiates the capacity of CCL39 cells to stabilize ascorbate. We discuss here the role of ascorbate free radical on growth control by its reduction by the plasma membrane redox system and its meaning for cell physioslogy.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Alcaín FJ, Burón MI, Rodríguez-Aguilera JC, Villalba JM, Navas P (1990) Ascorbate free radical stimulates the growth of a human promyelocytic leukemia cell line. Cancer Res 50: 5887–5891
— —, Villalba JM, Navas P (1991) Ascorbate is regenerated by HL-60 cells through the transplasma membrane redox system. Biochim Biophys Acta 1073: 380–385
Anderson R, Oosthuizen R, Maritz R, Theron A, van Rensbur AJ (1980) The effects of increasing weekly doses of ascorbate in certain cellular and humoral immune functions in normal volunteers. Am J Clin Nutr 33: 71–76
Crane FL, Sun IL, Clark MG, Grebing C, Löw H (1985) Transplasma membrane redox systems in growth and development. Biochim Biophys Acta 811: 233–264
Gardiner NS, Duncan JR (1989) Inhibition of murine melanoma growth by sodium ascorbate. J Nutr 119: 586–590
Goldenberg H, Grebing C, Löw H (1983) NADH-monodehydroascorbate reductase in human erythrocyte plasma membrane. Biochem Int 6: 1–10
González-Quevedo M, Alcaín FJ, González-Reyes JA. Burón MI, Navas P (1991) Growth patterns of the HL-60 human promyelocytic cell line stimulated by ascorbate free radical. Cancer J 4: 262–266
González-Reyes JA, Döring O, Navas P, Obst G, Böttger M (1992) The effect of ascorbate free radical on the energy state of the plasma membrane of onion (Allium cepa L.) root cells: alteration of K+ efflux by ascorbate? Biochim Biophys Acta 1098: 177–183
—, Hidalgo A, Caler JA, Palos R, Navas P (1994) Nutrient uptake changes in ascorbate free radical-stimulated onion roots. Plant Physiol 104: 271–276
Jacob RA, Pianalto FS, Agee RE (1992) Cellular ascorbate depletion in healthy men. J Nutr 122: 1111–1118
Jacobson EL, Jacobson MK (1976) Pyridine nucleotide levels as a function of growth in normal and transformed 3T3 cells. Arch Biochem Biophys 175: 627–634
Kobayashi K, Harada Y, Hayashi K (1991) Kinetic behavior of the monodehydroascorbate radical studied by pulse radiolysis. Biochemistry 30: 8310–8315
Matsumara M, Miyachi S (1980) Cycling assay for nicotinamide adenine dinucleotides. Methods Enzymol 69: 465–470
Medina MA, Schweigerer L (1993) A plasma membrane redox system in human retinoblastoma cells. Biochem Mol Biol Int 29: 881–887
—, Castillo-Olivares A, Schweigerer L (1992) Plasma membrane redox activity correlates with N-myc expression in neuroblastoma cells. FEBS Lett 311: 99–101
Morré DJ, Crane FL, Sun IL, Navas P (1987) The role of ascorbate in biomembrane energetics. Ann NY Acad Sci 498: 153–171
Navas P, Burón MI (1990) Roles of pyridine nucleotides in the control of cell growth. In: Crane FL, Morré DJ, Löw H (eds) Oxidoreduction at cell membranes: relation to growth and transport. CRC Press, Boca Raton, pp 225–236
—, Sun IL, Morré DJ, Crane FL (1986) Decrease of NADH in HeLa cells in the presence of transferrin or ferricyanide. Biochem Biophys Res Commun 135: 110–115
—, Estévez A, Burón MI, Villalba JM, Crane FL (1988) Cell surface glycoconjugates control the activity of the NADH-ascorbate free radical reductase of rat liver plasma membrane. Biochem Biophys Res Commun 154: 1029–1032
—, Alcaín FJ, Burón MI, Rodríguez-Aguilera JC, Villalba JM, Morré DM, Morré DJ (1992) Growth factor-stimulated trans plasma membrane electron transport in HL-60 cells. FEBS Lett 299: 223–226
—, Villalba JM, Córdoba F (1994) Ascorbate function at the plasma membrane. Biochim Biophys Acta 1197: 1–13
Park CH, Bergsagel DE, McCulloch EA (1971) Ascorbic acid: culture requirement for colony formation by mouse plasmacytoma cells. Science 174: 720–722
Park CM, Kimler BF (1991) Growth modulation of human leukemic, preleukemic and myeloma progenitor cells by L-ascorbic acid. Am J Clin Nutr 54: 12415–12465
Rodríguez-Aguilera JC, Navas P (1994) Extracellular ascorbate stabilization: enzymatic or chemical process? J Bioenerg Biomembr 26: 379–384
—, Navarro F, Arroyo A, Villalba JM, Navas P (1993) Transplasma membrane redox system of HL-60 cells is controlled by cAMP. J Biol Chem 268: 26346–26349
Schweinzer E, Goldenberg H (1992) Ascorbate-mediated trans-membrane electron transport and ascorbate uptake in leukemic cell lines are two different processes. Eur J Biochem 206: 807–812
Sun IL, Navas P, Crane PL, Chou JY, Löw H (1986) Transplasmalemma electron transport is changed in simian virus 40 transformed liver cells. J Bioenerg Biomembr 18: 471–485
Tsao CS, Dunham WB, Leung PY (1992) Effect of ascorbic-acid and its derivatives on the growth of human mammary-tumor xenografts in mice. Cancer J 5: 53–59
Tsao MS, Sanders GHS, Grisham JW (1987) Regulation of growth of cultured hepatic epithelial cells by transferrin. Exp Cell Res 171: 52–62
Winkler BS (1987) In vitro oxidation of ascorbic acid and its prevention by GSH. Biochim Biophys Acta 925: 258–264
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Navas, P., Gómez-Díaz, C. Ascorbate free radical and its role in growth control. Protoplasma 184, 8–13 (1995). https://doi.org/10.1007/BF01276895
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01276895