Summary
Changes in an autophagic system during differentiation of cells ofDictyostelium discoideum, NC-4 were studied under light and electron microscopes, and it was demonstrated cytochemically that acid phosphatase was almost exclusively localized in food and autophagic vacuoles. Autophagic vacuoles first appeared during formation of loose aggregates, coupled with the defecation of food vacuoles. Autophagic vacuoles seem to originate from flat sacs which segregate parts of the cytoplasm. No acid phosphatase was detected in the vacuoles when first formed, but activity appeared later probably due to fusion with Golgi-like vesicles. When starved cells were not allowed to aggregate due to a low cell density, they formed no autophagic vacuoles but retained many food vacuoles. This indicates that the formation of autophagic vacuoles is not simply due to starvation, but to cell interaction mediated by cell contact. Autophagic vacuoles containing acid phosphatase rapidly increased in number in all cells in the early stage of aggregation. After papillae formed, however, they selectively decreased in the prespore cells, but developed further and grew larger in the prestalk cells.
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Bonner, J. T., 1947: Evidence for the formation of cell aggregates by chemotaxis in the development of the slime moldDictyostelium discoideum. J. exp. Zool.106, 1–26.
—, 1952: The pattern of differentiation in amoeboid slime molds. Am. Nat.86, 79–89.
Chastellier de, C., Ryter, A., 1977: Changes of the cell surface and of the digestive apparatus ofDictyostelium discoideum during the starvation period triggering aggregation. J. Cell Biol.75, 218–236.
Ericsson, J. L. E., 1969: Mechanism of cellular autophagy. In: Lysosomes in biology and pathology, Vol.2 (Dingle, J. T., Fell, H. B., eds.), pp. 345–394. Amsterdam: North-Holland Publishing Company.
Francis, D., O'Day, D. H., 1971: Sorting out in pseudoplasmodia ofDictyostelium discoideum. J. exp. Zool.176, 265–272.
Gezelius, K., 1972: Acid phosphatase localization during differentiation in the cellular slime moldDictyostelium discoideum. Arch. Mikrobiol.85, 51–76.
Gomori, G., 1952: Microscopic histochemistry. Principles and practice. Chicago: Univ. of Chicago Press.
Hayashi, M., Takeuchi, I., 1976: Quantitative studies on cell differentiation during morphogenesis of the cellular slime moldDictyostelium discoideum. Devl. Biol.50, 302–309.
Hohl, H. R., Hamamoto, S. T., 1969: Ultrastructure of spore differentiation inDictyostelium: the prespore vacuole. J. Ultrastruct. Res.26, 442–453.
Ikeda, T., Takeuchi, I., 1971: Isolation and characterization of a prespore specific structure of the cellular slime mold,Dictyostelium discoideum. Devl. Growth Differ.13, 221–229.
MacWilliams, H. K., Bonner, J. T., 1979: The prestalk-prespore pattern in cellular slime molds. Differentiation14, 1–22.
Maeda, Y., Takeuchi, I., 1969: Cell differentiation and fine structures in the development of the cellular slime molds. Devl. Growth Differ.11, 232–245.
Newell, P. C., Longlands, M., Sussman, M., 1971: Control of enzyme synthesis by cellular interaction during development of the cellular slime moldDictyostelium discoideum. J. molec. Biol.58, 541–554.
Oohata, A., Takeuchi, I., 1977: Separation and biochemical characterization of the two cell types present in the pseudoplasmodium ofDictyostelium mucoroides. J. Cell Sci.24, 1–9.
Pitt, D., 1975: Lysosomes and cell function. London-New York: Longman Group Limited.
Ryter, A., Chastellier de, C., 1977: Morphometric and cytochemical studies ofDictyostelium discoideum in vegetative phase: digestive system and membrane turnover. J. Cell Biol.75, 200–217.
Strauss, A. J. L., Seegal, B. C., Hsu, K. C., Burkholder, P. M., Nastuk, W. L., Osserman, K. E., 1960: Immunofluorescence demonstration of a muscle binding, complement-fixing serum globulin fraction in myasthenia gravis. Proc. Soc. exp. Biol. Med.105, 184–191.
Takeuchi, I., 1963: Immunochemical and immunohistochemical studies on the development of the cellular slime moldDictyostelium mucoroides. Devl. Biol.8, 1–26.
—,Yabuno, K., 1970: Disaggregation of slime mold pseudoplasmodia using EDTA and various proteolytic enzymes. Exp. Cell Res.61, 183–190.
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Yamamoto, A., Maeda, Y. & Takeuchi, I. Development of an autophagic system in differentiating cells of the cellular slime moldDictyostelium discoideum . Protoplasma 108, 55–69 (1981). https://doi.org/10.1007/BF01276883
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DOI: https://doi.org/10.1007/BF01276883