Skip to main content
Log in

Dedifferentiation of chloroplasts in interspecific and homospecific protoplast fusion products

  • Published:
Protoplasma Aims and scope Submit manuscript

Summary

Interspecific and homospecific protoplast fusion products (Vicia narbonensis +V. hajastana,V. hajastana+V. hajastana) regenerated cell walls and divided when culturedin vitro for a period of 7 days. While most organelles appeared typical of actively growing plant cells, chloroplasts exhibited structural changes which were interpreted as dedifferentiation.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Chen, K., Johal, S., Wildman, S. G., 1977: Phenocypic markers for chloroplast DNA genes in higher plants and their use in biochemical genetics. In: Nucleic acids and protein synthesis in plants (Bogorad, L., Weil, J. W., eds.), pp. 183–194. New York: Plenum Press.

    Google Scholar 

  • Constabel, F., 1978: Development of protoplast fusion products, heterokaryocytes and hybrid cells. In: Frontiers of plant tissue culture 1978 (Thorpe, T. A., ed.), pp. 141–149. University of Calgary Press.

  • -Koblitz, H.,Kirkpatrick, J. W.,Rambold, S., 1979: Fusion of cell sap vacuoles subsequent to protoplast fusion. Can. J. Bot. In press.

  • —,Weber, G., Kirkpatrick, J. W., Pahl, K., 1976: Cell division of intergeneric protoplast fusion products. Z. Pflanzenphysiol.79, 1–7.

    Google Scholar 

  • Donn, G., 1978: Cell division and callus regeneration from leaf protoplasts ofVicia narbonensis. Z. Pflanzenphysiol.86, 65–75.

    Google Scholar 

  • Eriksson, T., 1965: Studies on the growth requirements and growth measurements of cell cultures ofHaplopappus gracilis. Physiol. Plant.18, 976–993.

    Google Scholar 

  • Fowke, L. C., 1975: Electron microscopy of protoplasts. In: Plant tissue culture methods (Gamborg, O. L., Wetter, L. R., eds.). National Research Council of Canada, Saskatoon.

    Google Scholar 

  • - 1978: Ultrastructure of isolated and cultured protoplasts. In: Frontiers of plant tissue culture 1978 (Thorpe, T. A., ed.), pp. 223–233. University of Calgary Press.

  • —,Constabel, F., Gamborg, O. L., 1977: Fine structure of fusion products from soybean cell culture and pea leaf protoplasts. Planta (Berl.)135, 257–266.

    Google Scholar 

  • —,Rennie, P. J., Kirkpatrick, J. W., Constabel, F., 1976: Ultrastructure of fusion products from soybean cell culture and sweet clover leaf protoplasts. Planta (Berl.)130, 39–45.

    Google Scholar 

  • Gamborg, O. L., Miller, R. A., Ojima, K., 1968: Nutrient requirements of suspension cultures of soybean root cells. Exp. Cell Res.50, 151–158.

    Google Scholar 

  • Gigot, C., Kopp, M., Schmitt, C., Milne, R. G., 1975: Subcellular changes during isolation and culture of tobacco mesophyll protoplasts. Protoplasma84, 31–41.

    Google Scholar 

  • Gunning, B. E. S., Steer, M. W., 1975: Ultrastructure and the biology of plant cells. London: E. Arnold.

    Google Scholar 

  • Kao, K. N., Constabel, F., Michayluk, M. R., Gamborg, O. L., 1974: Plant protoplast fusion and growth of intergeneric hybrid cells. Planta (Berl.)130, 215–227.

    Google Scholar 

  • Sjölund, R. D., Weier, T. E., 1971: An ultrastructural study of chloroplast structure and dedifferentiation in tissue cultures ofStreptanthus tortuosus (Cruciferae). Amer. J. Bot.58, 172–181.

    Google Scholar 

  • Weber, G., Constabel, F., Williamson, F., Fowke, L. C., Gamborg, O. L., 1976: Effect of preincubation of protoplasts on PEG-induced fusion of plant cells. Z. Pflanzenphysiol.79, 459–464.

    Google Scholar 

  • Williamson, F. A., Fowke, L. C., Weber, G., Constabel, F., Gamborg, O. L., 1977: Microfibril deposition on cultured protoplasts ofVicia hajastana. Protoplasma91, 213–220.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Rennie, P.J., Weber, G., Constabel, F. et al. Dedifferentiation of chloroplasts in interspecific and homospecific protoplast fusion products. Protoplasma 103, 253–262 (1980). https://doi.org/10.1007/BF01276271

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01276271

Keywords

Navigation