Summary
The electron-microscopic localization of ATPase activity was studied in the median lateral bud-bearing nodes in intact and decapitated sporophytes ofMarsilea drummondii. ATPase activity was confined to the plasma membrane of xylem transfer cells, phloem parenchyma cells and sieve tubes belonging to the stele of the main axis. No activity was found in the bud branch. One of the membrane-bound ATPase components is a Mg2+ ATPase, which is stimulated by K+ and functions optimally at pH 6.5 with ATP as substrate. Numerous inhibitors were tested which restricted or completely inhibited ATPase sites in transfer cells and sieve tubes. The use of CCCP, DCCD, DES, NEM or PCMBS allowed a distinction to be made between these conducting elements which are differentially affected by the inhibitors. The structurally polarized transfer cells remain functional on the side abutting the xylem tracheids but are inoperative on the opposite side,i.e., in phloem loading. The Mg2+-K+ ATPase in the bud trace was rapidly stimulated (in one hour) after decapitation. These results are consistent with a transport role for this ATPase, and support the view that its stimulation is a prerequisite for the resumption of bud activity.
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Abbreviations
- ATP, ADP, and AMP (as the disodium salts):
-
adenosine tri-5′, di-5′, and mono-5′-phosphate
- UTP:
-
uridine triphosphate
- βGP:
-
sodium β glycerophosphate
- p-NPP:
-
para-nitrophenyl phosphate
- CCCP:
-
carbonyl-cyanide-m-chlorophenyl-hydrazone
- DCCD:
-
N.N′-dicyclohexylcarbodiimide
- DES:
-
diethylstilbestrol
- DNP:
-
2,4 dinitrophenol
- NAF:
-
sodium fluoride
- NEM:
-
N-ethylmaleimide
- PCMBS:
-
p-chloromercuribenzene sulfonic acid
- TRIS:
-
Tris (hydroxymethyl) methylamine
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This study was supported by a grant from the Centre National de la Recherche Scientifique.
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Sossountzov, L., Habricot, Y. Ultracytochemical localization and characterization of membrane-bound ATPases in lateral buds from intact and decapitated plants of an aquatic fern,Marsilea drummondii A. Br.. Protoplasma 127, 180–191 (1985). https://doi.org/10.1007/BF01276262
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DOI: https://doi.org/10.1007/BF01276262