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Plantlet regeneration from mesophyll protoplasts ofBetula platyphylla var.japonica

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Summary

InBetula platyphylla var.japonica, colonies were induced efficiently from mesophyll protoplasts cultured in half strength MS (1/2MS) liquid medium containing 0.6 M mannitol, 0.09M sucrose and 1 μM 4-PU and 1 μM NAA at a cell density of 5 × 104/ml. The colonies grew actively and developed into callus after 3 months of culture.

Roots differentiated from the protoplast-derived white calluses cultured on the 1 /2MS solid media supplemented with 0.1–1 μM 4-PU and 1 μM NAA, and 10 μM zeatin with no supplementation of NAA. Furthermore, the protoplast-derived green callus differentiated shoots with 1/2MS solid medium containing 1 μM 4-PU or 10 μM zeatin with no supplementation of NAA. When shoots obtained were cultured on the cytokinin-free MS solid medium with 2.5 μM IBA and 0.1 μM NAA, they rooted and developed into plantlets after one month of culture.

The phenylurea-type cytokinin, 4-PU, was effective for plantlet regeneration from the mesophyll protoplasts ofB. platyphylla var.japonica. This suggests that there is potential for the use of 4-PU in the culture of protoplasts in many forest tree species.

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Abbreviations

BAP:

6-benzylaminopurine

2,4-D:

2,4-dichlorophenoxyacetic acid

FDA:

fluorescein diacetate

IBA:

indole-3-butyric acid

2ip:

N 6-(2-isopentenyl)-adenine

NAA:

1-naphthaleneacetic acid

4-PU:

N-(2-chloro-4-pyridyl)-N−phenylurea

TDZ:

thidiazuron

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Authors and Affiliations

  1. Forestry and Forest Products, Department of Forest Science, Faculty of Agriculture, Utsunomiya University, Utsunomiya 321, Tochigi, Japan

    Yohichi Wakita, Shinso Yokota & Nobuo Yoshizawa

  2. Forestry and Forest Products Research Institute, Ministry of Agriculture, Forestry and Fisheries, Tsukuba 305, P.O. Box 16, Ibaraki, Japan

    Hamako Sasamoto

Authors
  1. Yohichi Wakita
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  2. Hamako Sasamoto
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  3. Shinso Yokota
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  4. Nobuo Yoshizawa
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Communicated by A. Komamine

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Wakita, Y., Sasamoto, H., Yokota, S. et al. Plantlet regeneration from mesophyll protoplasts ofBetula platyphylla var.japonica . Plant Cell Reports 16, 50–53 (1996). https://doi.org/10.1007/BF01275448

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  • DOI: https://doi.org/10.1007/BF01275448

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