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Ultrastructure ofCatharanthus roseus cells culturedin vitro and exposed to conditions for alkaloid accumulation

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Summary

Periwinkle (Catharanthus roseus) cells cultured in 1-B 5 medium display the ultrastructure of parenchyma cells. The parenchyma character remained unchanged when cells were exposed to any one of three different conditions effecting alkaloid accumulation. Transfer of cells to “alkaloid production medium” for 2 weeks (condition 1) accorded two special features,i.e., unusually big lipid droplets in the cytoplasm and, upon fixation, one or several electron-dense droplets of spongy precipitate in vacuoles. Among hormone-autotrophic cultures (condition 2) some cells showed a fine electron-dense vacuolar precipitate. Addition ofPhythium homogenate (fungal elicitor) to cells cultured in 1-B 5-medium for 10 days (condition 3), cells showed a frequent appearance of singular big lipid droplets in the cytoplasm, whereas vacuoles remained devoid of precipitate. The appearance of big lipid droplets and of vacuolar precipitate is interpreted as progressing cytodifferentiation, but is coincidental with alkaloid accumulation.

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References

  • Balsevich J (1987) Monoterpene indole alkaloids from apocynaceous plants other thanCatharanthus roseus. In:Constabel F (ed) Cell culture and somatic cell genetics of plants, vol 5. Academic Press, New York

    Google Scholar 

  • DeLuca V, Kurz WGW (1987) Monoterpene indole alkaloids (Catharanthus alkaloids). In:Constabel F (ed) Cell culture and somatic cell genetics of plants, vol 5. Academic Press, New York

    Google Scholar 

  • Deus-Neumann B, Zenk MH (1984) A highly selective alkaloid uptake system in vacuoles of higher plants. Planta 162: 250–260

    Google Scholar 

  • Eilert U, Constabel F (1985) Ultrastructure ofPapaver somniferum cells culturedin vitro and treated with fungal homogenate eliciting alkaloid production. Protoplasma 128: 38–42

    Google Scholar 

  • —,Nesbitt LR, Constabel F (1985) Laticifers and latex in fruits of periwinkle,Catharanthus roseus. Can J Bot 63: 1540–1546

    Google Scholar 

  • —,Constabel F, Kurz WGW (1986) Elicitor-stimulation of monoterpene indole alkaloid formation in suspension cultures ofCatharanthus roseus. J Plant Physiol 126: 11–22

    Google Scholar 

  • -DeLuca V,Kurz WGW,Constabel F (1987) Alkaloid formation by habituated and tumorous cell suspension cultures ofCatharanthus roseus. Plant Cell Reports

  • Gamborg OL, Miller RA, Ojima K (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res 50: 151–158

    PubMed  Google Scholar 

  • Kurz WGW, Chatson KB, Constabel F, Kutney JP, Choi LSL, Kolodziejczyk P, Sleigh SK, Stuart KL, Worth B (1981) Alkaloid production inCatharanthus roseus cell cultures. III. Characterization of the PRL no. 200 cell line. Planta Med 46: 22–31

    Google Scholar 

  • —,Constabel F (1982) Production and isolation of secondary metabolites. In:Wetter LR, Constabel F (eds) Plant tissue culture methods, 2nd edn. National Research Council of Canada, Ottawa, NRCC no 19876

    Google Scholar 

  • - (1984) Variations in indole alkaloid accumulation in cell suspension cultures fromCatharanthus roseus cultivars. Kyoto Symposium on Bioscience: Prospects in Plant Cell Science and Technology, Kyoto, Japan, pp 11–20

  • Mersey BG, Cutler AJ (1986) Differential distribution of specific indole alkaloids in leaves ofCatharanthus roseus. Can J Bot 64: 1039–1045

    Google Scholar 

  • Neumann D, Krauss G, Hicke M, Gröger D (1983) Indole alkaloid formation and storage in cell suspension cultures ofCatharanthus roseus. Planta Med 48: 20–23

    Google Scholar 

  • Zenk MH, El-Shagi H, Arens H, Stöckigt J, Weiler EW, Deus B (1977) Formation of the indole alkaloids serpentine and ajmalicine in cell suspension cultures ofCatharanthus roseus. In:Barz W, Reinhard E, Zenk MH (eds) Plant tissue culture and its bio-technological application. Springer, Berlin Heidelberg New York, pp 27–43

    Google Scholar 

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Authors and Affiliations

  1. Plant Biotechnology Institute, National Research Council of Canada, S7N/OW9, Saskatoon, Sask.

    Udo Eilert (Visiting Scientist), Wolfgang G. W. Kurz & Friedrich Constabel

  2. Institut f. Pharm. Biologie, Universität Braunschweig, Braunschweig, Germany

    Udo Eilert (Visiting Scientist)

Authors
  1. Udo Eilert
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  2. Wolfgang G. W. Kurz
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  3. Friedrich Constabel
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NRCC no. 24524.

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Eilert, U., Kurz, W.G.W. & Constabel, F. Ultrastructure ofCatharanthus roseus cells culturedin vitro and exposed to conditions for alkaloid accumulation. Protoplasma 140, 157–163 (1987). https://doi.org/10.1007/BF01273725

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  • DOI: https://doi.org/10.1007/BF01273725

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