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Turgor regulation and cytoplasmic free Ca2+ in the algaLamprothamnium

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Summary

In internodal cells ofLamprothamnium succinctum, turgor regulation in response to hypotonie treatment is inhibited by lowering external Ca2+ concentration ([Ca2+]e) from 3.9 (normal) to 0.01 (low) mM. In order to clarify whether a change in the cytoplasmic free Ca2+ concentration ([Ca2+]c) is involved in turgor regulation, the Ca2+ sensitive protein aequorin was injected into the cytoplasm of internodal cells. A large transient light emission was observed upon hypotonic treatment under normal [Ca2+]e but not under low [Ca2+]e. Thus hypotonic treatment induces a transient increase in [Ca2+]c under normal [Ca2+]e but not under low [Ca2+]e.

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Abbreviations

ASW:

artificial sea water

πi :

cellular osmotic pressure

[Ca2+]c :

cytoplasmic free Ca2+ concentration

EDTA:

ethylenediamine-tetraacetic acid

EGTA:

ethylenglycol-bis(β-aminoethyl ether(N,N′-tetraacetic acid

[Ca2+]e :

external Ca2+ concentration

πe :

external osmotic pressure

GM:

glass micropipette

GP:

glass plate

HEPES:

N-2-hydroxyethylpiperazine-N′-2-ethansulfonic acid

MS:

microscope stage

OL:

objective lens

PIPES:

piperazine-N-N′-bis(2-ethanesulfonic acid)

W:

Weight

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Author notes

  1. Yoshiji Okazaki

    Present address: Department of Biology, Osaka Medical College, Sawaragi-cho 2-41, 569, Osaka, Japan

  2. Yasuaki Yoshimoto & Yukio Hiramoto

    Present address: The University of the Air, Wakaba 2-11, 260, Chiba, Japan

Authors and Affiliations

  1. Department of Biology, Faculty of Science, University of Tokyo, Hongo 7-3-1, 113, Tokyo, Japan

    Yoshiji Okazaki, Yasuaki Yoshimoto, Yukio Hiramoto & Masashi Tazawa

  2. Department of Cell Biology, National Institute for Basic Biology, Okazaki, 444, Aichi, Japan

    Yoshiji Okazaki, Yasuaki Yoshimoto, Yukio Hiramoto & Masashi Tazawa

  3. Biological Laboratory, Tokyo Institute of Technology, 152, Tokyo, Japan

    Yoshiji Okazaki, Yasuaki Yoshimoto, Yukio Hiramoto & Masashi Tazawa

Authors
  1. Yoshiji Okazaki
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  2. Yasuaki Yoshimoto
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  3. Yukio Hiramoto
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  4. Masashi Tazawa
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Okazaki, Y., Yoshimoto, Y., Hiramoto, Y. et al. Turgor regulation and cytoplasmic free Ca2+ in the algaLamprothamnium . Protoplasma 140, 67–71 (1987). https://doi.org/10.1007/BF01273256

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  • DOI: https://doi.org/10.1007/BF01273256

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