Summary
To control the intracellular free Ca2+ concentration from the cell exterior, pollen tubes ofLilium longiflorum were treated with a Ca2+ ionophore, A23187. Cytoplasmic streaming was inhibited when the free Ca2+ concentration of the external medium ([Ca2+]) was raised to 5×10−6 M or higher. At [Ca2+] below 1×10−6 M, the rhodamine-phalloidin stained actin filaments appeared straight and thin. However, at [Ca2+] which inhibited cytoplasmic streaming, the actin filaments appeared fragmented. In pollen tubes, Ca2+ regulation of cytoplasmic streaming may be linked not only to myosin (Shimmen 1987) but also to actin.
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Abbreviations
- ATP:
-
adenosine-5′-triphosphoric acid
- [Ca2+]:
-
concentration of free Ca2+
- EGTA:
-
ethyleneglycol-bis-(β-aminoethylether)N,N,N,N′-tetraacetic acid
- HEPES:
-
N-2-hydroxyethylpiperazine-N′-2-ethanesulfonic acid
- PIPES:
-
piperazine-N,N′-bis(2-ethanesulfonic acid)
- Rh-ph:
-
rhodamine-conjugated phalloidin
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Kohno, T., Shimmen, T. Ca2+-induced fragmentation of actin filaments in pollen tubes. Protoplasma 141, 177–179 (1987). https://doi.org/10.1007/BF01272900
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DOI: https://doi.org/10.1007/BF01272900