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A new cell line from human undifferentiated carcinoma of the ovary: establishment and characterization

  • Original Paper
  • Experimental Oncology
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Abstract

A cell line designated CUMO-2 has been established from an undifferentiated ovarian carcinoma. The s.c. injection of cells into nude mice gave rise to fast-growing tumors, while the i.p. route induced a peritoneal carcinomatosis with ascites. Histopathologically, the transplanted s.c. tumors closely resembled the original tumor, but tumors developed in the peritoneal cavity were highly anaplastic. The epithelial nature of the cells was confirmed by ultrastructural analysis. Sequential cytogenetic analyses on early and late passages revealed highly aneuploid tumor cells with consistent structural aberrations of chromosomes 1, 3, 8 and 11. CUMO-2 cells were found to produce CA 125 in vitro and in vivo. Cytosol estrogen receptor (ER) was found but progesterone receptor (PR) was not measured. HLA typing indicated the presence of DR8 and DQw4. A gonadotropin-releasing hormone (Gn-RH) analog inhibited cell growth and Gn-RH receptor mRNA was detected by reverse transcription/polymerase chain reaction in this cell line. Administration of transforming growth factor β1 inhibited both cell growth and c-myc mRNA expression. This cell line demonstrated a conformational band shift in exon 7 of thep53 gene. It was a frameshift mutation.

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Abbreviations

Gn-RH :

gonadotropin-releasing hormone

ER :

estrogen receptor

PR :

progesterone receptor

RT-PCR :

reverse transcription/polymerase chain reaction

TGF :

transforming growth Factor

SSCP :

Single-strand conformation polymorphism

MTT :

3-(4,5-dimethylthiazol-2-yl)-2,5-diphenytetrazolium bromide

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Authors and Affiliations

  1. Department of Obstetrics and Gynecology, Kangnam St. Mary's Hospital Catholic Cancer Center, Catholic University Medical College, 505 Banpo-dong, Seocho-ku, 137-040, Seoul, Korea

    Jin Woo Kim, Heung Kee Kim, Jong Gu Rha, Dae Hoon Kim, Seung Jo Kim & Sung Eun Namkoong

  2. Department of Medical Genetics, Hanyang University School of Medicine, 133-791, Seoul, Korea

    Chun Geun Lee & Myung Soo Lyu

Authors
  1. Jin Woo Kim
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  2. Chun Geun Lee
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  3. Myung Soo Lyu
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  4. Heung Kee Kim
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  5. Jong Gu Rha
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  6. Dae Hoon Kim
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  7. Seung Jo Kim
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  8. Sung Eun Namkoong
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This work was supported by a grant from the Korea research foundation, 1990

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Kim, J.W., Lee, C.G., Lyu, M.S. et al. A new cell line from human undifferentiated carcinoma of the ovary: establishment and characterization. J Cancer Res Clin Oncol 123, 82–90 (1997). https://doi.org/10.1007/BF01269885

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  • DOI: https://doi.org/10.1007/BF01269885

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