Summary
It has been found that certain mutants of TMV with known amino acid exchanges produce abnormally low quantities of infectious virus particlesin vivo at 32°C, although the particles themselves are stable at even higher temperatures. A correlation between temperature sensitivity of the mutantsin vivo and the inability of their A-Proteins to reaggregate to rodsin vitro at 30°C is demonstrated. It is concluded that the amino acid exchanges in coat protein lead to temperature sensitivity of the mutants described.
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Jockusch, H. In vivo- undin vitro-verhalten temperatursensitiver mutanten des Tabakmosaikvirus. Zeitschrift für Vererbungslehre 95, 379–382 (1964). https://doi.org/10.1007/BF01268671
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DOI: https://doi.org/10.1007/BF01268671