Summary
Experiments in vitro have been conducted to determine the surface-charge density of human red cells incubated with 7 quartz varieties as well as the UICC asbestos dusts: chrysotiles A and B, anthophyllite, amosite, and crocidolite. Red cell mobility was measured by capillary migration on Whatman paper strips and by electrophoresis. All dusts tested significantly decreased the erythrocyte surface electric charge as compared to control samples that were free of dust.
Pretreatment of quartz dust with Polyvinylpyridine-N-Oxide restored the electronegativity of erythrocytes. The results obtained provide support for the cytotoxic effect of quartz and asbestos on the external cell membrane.
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References
Ambrose, E.J.: Electrophoretic behaviour of cells. Progr.Biophys.Mol.Biol.16, 241–265 (1966)
Bäckers, V.H., Robock, K., Klosterkotter, W.: Untersuchungen über die zelluläre Schutzwirkung von Polyvinylpyridin-N-Oxid gegenaber dem Standardquartz DQ 12. Ergebnisse von Untersuchungen auf dem Gebiet der Staub- und Silikosebekämpfung im Steinkohlenbergbau, Bd.9, S.ß91–98. Essen: Glückauf 1973
Cook, G.M.W., Heard, D.H., Seaman, G.V.F.: Sialic acids and the electrokinetic charge of the human erythrocytes. Nature191, 44–49 (1961)
Cook, G.M.W., Jacobson, W.: The electrophoretic mobility of normal and leukaemic cells of mice. Biochem.J.107, 549–557 (1968)
Dalton, A.J., Haguenau, F.: Ultrastructure in biological systems. The membranes. New York - London: Academic Press 1968
Durocher, J.R., Glader, B.E., Conrad, M.E.: Effects of cyanate on erythrocyte membrane surface charge. Proc.Soc.Exp.Biol.Med.144, 249–251 (1973)
Eylar, H., Madoff, M.A., Brody, O.V., Oucley, Z.L.: The contribution of sialic acid to the surface charge of the erythrocyte. J.Biol.Chem.237, 1992–2000 (1962)
Ganzer, E., Fuhrman, G.F., Ruhenstroth-Bauer, G.: Untersuchungen aber die mit Neuraminidase abspaltbaren Neuraminsaurederivate aus Oberflächenmembranen normaler Leberzellen und Asciteshepatomzellen von Ratten. Hoppe-Seyler's Z.Physiol.Chem.337, 52–56 (1967)
Haydon, D.A., Seaman, G.V.F.: Electrokinetic studies on the ultrastructure of the human erythrocyte. Arch.Biochem.Biophys.122, 126–136 (1967)
HiroshiNagura, Jumpei Asai, Yoshinao Katsumata, Kiyohide Kojima: Role of electric surface charge of cell membrane in phagocytosis. Acta.Path. Jap.23, 279–290 (1973)
Hounan, R.F.: Some reactions between inert dusts and proteins. AMA Arch. Industr.Hyg.Occup.Med.9, 284–290 (1954)
Kosztolányi, G., Kellermayer, N., Jobst, K.: On the surface potential of pneumoconiotic dusts. Beitr.Path.147, 42–44 (1972)
Lichtman, M.A., Weed, R.I.: Electrophoretic mobility and N-acetyl neuraminic acid content of human normal and leukemic lymphocytes and granulocytes. Blood35, 12–22 (1970)
Mehrishi, J.N., Seaman, G.V.F.: Temperature dependence of the electrophoretic mobility of cells and quartz particles. Biochim.Biophys.Acta112, 154–159 (1966)
Metzger, G.V., Casarett, L.J.: Some effects of divalent cations on in vitro phagocytosis. In: Reticuloendothelial systems and atherosclerosis, pp.163–174. New York: Plenum Press 1967
Nash, T., Allison, A.C., Harington, J.S.: Physico-chemical properties of silica in relation to its toxicity. Nature210, 259–261 (1966)
Pott, F., Schlipketer, N.W., Brockhaus, A.: Tierexperimentelle Grundlagen zur medizinischen Silikoseprophylaxie mit Polyvinylpyridin-N-Oxid (P 204). Dtsch.Med.Wschr.93, 2479–2482 (1969)
Robock, K.: A new concept of the pathogenesis silicosis. Luminiscence measurements and biochemical cell experiments with SiO2 dusts. StaubReinhalt.Luft38, 15–26 (1968)
Robock, K., Beck, E.G., Manojlovic, N.: Sofortreaktion von Makrophagen auf SiO2-Stäubein vitro. Ergebnisse von Untersuchungen auf dem Gebiet der Staub- und Silikosebekämpfung im Steinkohlen-Bergbau, Bd.9, S.91–98. Essen: Glückauf 1973
Schlipköter, N.W.: Posibilities of causal prophylaxis and therapy of pneumoconiosis. Arch.Environm.Hlth.21, 181–191 (1970)
Weiss, L., Zeigel, R.: Cell surface negativity and the binding of positively charged particles. J.Cell.Physiol.77, 179–183 (1971)
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Gabor, S., Frits, T. & Anca, Z. Effect of quartz and asbestos on erythrocyte surface charge. Int. Arch Occup Environ Heath 36, 47–55 (1975). https://doi.org/10.1007/BF01267851
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DOI: https://doi.org/10.1007/BF01267851