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Ultrastructural cytology of pea leaves and roots infected by pea seedborne mosaic virus

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Summary

Eleven isolates of pea seedborne mosaic virus (PSbMV) from U.S. seedlots were compared by thin-section electron microscopy to appraise the cytopathological diversity among isolates. Nine isolates uniformly induced formation of typical pinwheel inclusions, two of these also inducing tonoplast aggregates (TA) in a few cells. One isolate produced principally TA, accompanied in a few cells by pinwheel inclusions. The remaining isolate induced formation of dense bodies and laminated aggregates like those reported for bean yellow mosaic virus (Kamei et al., 1969). Other cytological abnormalities induced by one or more PSbMV isolates included extensive masses of convoluted endoplasmic reticulum, aggregates of viruslike particles, and crystalline inclusions consisting of ultrastructural hexagons. Symptoms induced by the eleven isolates in seedlings from which they originated fell within the range previously reported for this virus (Hampton, 1972). Root parenchyma cells from PSbMV-infected plants contained few inclusions, but instead contained extensive masses of viruslike particles that occupied most of the cytoplasmic volume in some cells. Samples of purified virus prepared separately from infected root and leaf tissues were morphologically indistinguishable.

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Author notes

  1. R. O. Hampton

    Present address: Research Plant Pathologist and Biological Aid, Western Region, United States Department of Agriculture, USA

  2. J. E. Knesek

    Present address: Department of Virology, University of Texas, in D. Anderson Hospital and Tumor Institute, 77025, Houston, TX, USA

Authors and Affiliations

  1. Research Plant Pathologist and Biological Aid, Western Region, United States Department of Agriculture, USA

    Sandra Phillips, J. E. Knesek & G. I. Mink

  2. Department of Botany and Plant Pathology, Oregon State University, Corvallis, Oregon, USA

    R. O. Hampton, Sandra Phillips, J. E. Knesek & G. I. Mink

  3. Irrigated Agriculture Research and Extension Center, Washington State University, 99350, Prosser, WA, USA

    G. I. Mink

Authors
  1. R. O. Hampton
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  2. Sandra Phillips
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  3. J. E. Knesek
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  4. G. I. Mink
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Additional information

Oregon Agricultural Experiment Station, Technical Paper No. 3441.

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Hampton, R.O., Phillips, S., Knesek, J.E. et al. Ultrastructural cytology of pea leaves and roots infected by pea seedborne mosaic virus. Archiv f Virusforschung 42, 242–253 (1973). https://doi.org/10.1007/BF01265649

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  • DOI: https://doi.org/10.1007/BF01265649

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