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A cytophotometric study of chromosome pairing

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Summary

  1. 1.

    Confirming earlier studies (Schrader 1945a and b), the chromosomes of the harlequin lobe of the testis of the pentatomid Loxa flavicolis are found to be asynaptic throughout late meiotic prophase and to divide equationally at metaphase I. All other lobes show a normal meiosis.

  2. 2.

    Some new evidence is presented for a reduction in chromosome number in the second meiotic division of the harlequin lobe.

  3. 3.

    Cytophotometric determination of relative amounts of DNA (Feulgen) and histones (Alfert-Geschwind Fast Green) in nuclei of normal and asynaptic lobes show that asynapsis is correlated with a 3/2 ratio of histone to DNA.

  4. 4.

    The 3/2 ratio is interpreted not as an increased amount of histone but as a change in the chemical union between histone and DNA, or alternatively with a change in the kind of histone present.

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References cited

  • Alfert, M.: Changes in the staining capacity of nuclear components during cell degeneration. Biol. Bull.109, 1–12 (1955a).

    Google Scholar 

  • — Cytochemical detection of a difference in protein composition between micronucleus and macronucleus ofTetrahymena pyriformis. Anat. Rec.122, 428–429 (1955b).

    Google Scholar 

  • Alfert, M., andI. Geschwind: A selective staining method for the basic proteins of cell nuclei. Proc. Nat. Acad. Sci. U.S.A.39, 991–995 (1953).

    Google Scholar 

  • Ansley, H. R.: A cytological and cytophotometric study of alternative pathways of meiosis in the house centipede (Scutigera forceps Rafinesque). Chromosoma6, 656–695 (1954).

    PubMed  Google Scholar 

  • Beadle, G. W.: Genetical and cytological studies of Mendelian asynapsis inZea mays. Memoir 129, Agricult. Exper. Station, Cornell Univ. 1930.

  • Bloch, D. P., andG. C. Godman: Evidence of differences in the desoxyribonucleoprotein complex of proliferating and non-dividing cells. J. Biophys. a. Biochem. Cytology1, 531–550 (1955).

    Google Scholar 

  • Darlington, C. D., andT. Dobzhansky: Temperature and “sex ratio” inDrosophila pseudoobscura. Proc. Nat. Acad. Sci. U.S.A.28, 45–48 (1942).

    Google Scholar 

  • Pollister, A. W.: Nucleoproteins of the nucleus. Exper. Cell Res. Suppl.2, 59–74 (1952).

    Google Scholar 

  • Schrader, F.: Regular occurrence of heteroploidy in a group of Pentatomidae (Hemiptera). Biol. Bull.88, 63–70 (1945a).

    Google Scholar 

  • —: The cytology of regular heteroploidy in the genusLoxa (Pentatomidae-Hemiptera). J. of Morph.76, 157–178 (1945b).

    Google Scholar 

  • Smith, L.: Cytogenetics of a factor for multiploid sporocytes in barley. Amer. J. Bot.29, 451–456 (1942).

    Google Scholar 

  • Stedman, E., andE. Stedman: Cell specificity of histones. Nature (Lond.)166, 780–781 (1950).

    Google Scholar 

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Authors and Affiliations

  1. Department of Biology, The Johns Hopkins University, Baltimore

    Hudson Ansley

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  1. Hudson Ansley
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Ansley, H. A cytophotometric study of chromosome pairing. Chromosoma 8, 380–395 (1956). https://doi.org/10.1007/BF01259510

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  • DOI: https://doi.org/10.1007/BF01259510

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