Summary
The effect of purified toxin obtained from the venom of the Brazilian scorpionTityus serrulatus on the small and large granular vesicles and on the large agranular vesicles of the Auerbach's plexus of the rat was investigated. Two segments of the rat ileum were immersed in an isolated organ bath containing Jalon's solution and toxin (15μg/ml) was added to one of these baths (9 exps.). The vesicles were counted in 1300 transversely cut axons and the number of vesicles per 100μ 2 of axonal area was estimated. The toxin did not produce any change in the morphology of the vesicles, but differences in the distribution of the vesicles were found. In the treated group a decrease in the number of the granular (dense) vesicles and an increase in the number of the agranular vesicles was observed. Our findings suggest that granular vesicles contain catecholamines and substance P, and that scorpion toxin by releasing both substances, decreases the number of these vesicles.
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References
Burn, J. H., D. J. Finney, andL. G. Goodwin: Biological standartization, sec. edition, p. 223. London: Oxford University Press. 1950.
Burnstock, G.: Purinergic nerves. Pharmacol. Rev.24, 509–581 (1972).
Cunha-Melo, J. R., L. Freire-Maia, W. L. Tafuri, andT. A. Maria: Mecanismo de açíe da toxina purificada de escorpião sobre o ileo isolado do rato. Acta physiol. latinoam.20, 296 (1970).
Cunha-Melo, J. R., L. Freire-Maia, W. L. Tafuri, andT. A. Maria: Mechanism of action of purified scorpion toxin on the isolated rat intestine. Toxicon11, 81–84 (1973).
De Robertis, E., andH. S. Bennet: Submicroscopic vesicular component in the synapse. Fed. Proc.13, 35 (1954).
Diniz, C. R., andJ. M. Torres: Release of acetylcholine like substance from guinea-pig ileum by scorpion venom. Toxicon8, 277–281 (1970).
Diniz, C. R., andJ. M. Gonçalves: Some chemical and pharmacological properties of Brazilian scorpion venoms. In: Venoms (Buckley, E. E., andN. Porges, eds.), p. 131–139. Washington: Am. Assoc. Adv. Sci. Pub. 1956.
Freire-Maia, L., J. R. Cunha-Melo, andM. V. Gomez: Tityustoxina, instrumente para estudo da fisiologia da musculatura lisa. Cienc. Cult. (São Paulo)25 (suppl.), 279–280 (1973).
Freire-Maia, L., andC. R. Diniz: Pharmacological action of a purified scorpion toxin in the rat. Toxicon8, 132 (1970).
Gomez, M. V., andC. R. Diniz: Separation of toxic components from Brazilian scorpion —Tityus serrulatus — venom. Mem. Inst. Butantan (São Paulo)33, 899–902 (1966).
Grillo, M., andS. L. Palay: Granule containing vesicles on the autonomie nervous system. In: Proceedings of the 5th International Congress of the Electron Microscopy (Breese, S. S., ed.), Vol. 2, U-1. New York: Academic Press. 1962.
Hager, H., andW. L. Tafuri: Elektronenoptische Nachweise sogenannter neurosekretorischer Elementargranula in marklosen Nervenfasern des plexus myentericus (Auerbach) des Meerschweinchens. Naturwissenschaften46, 333–334 (1959).
Henriques, M. C., C. R. Diniz, andM. V. Gomez: Estudos bioquímicos do processo de liberação da acetilcolina nos plexos nervosos do sistema nervoso autonomo. Paper presented to the Society of Biology of Minas Gerais, Belo Horizonte, Brazil, October 29, 1971.
Henriques, M. C., C. R. Diniz, andM. V. Gomez: Liberação de acetilcolina do ileo da cobaia por tityustoxina. Cienc. Cult. (São Paulo)25 (suppl.), 150 (1973).
Hial, W., andC. R. Diniz: Efeito da Scorpiotoxina sobre o conteudo de substancia P do intestine de rato. Cienc. Cult. (São Paulo)23 (suppl.), 304 (1971).
Hial, W., C. R. Diniz, J. E. H. Patella, andW. L. Tafuri: Quantitative study of P substance in the megaesophagus and megacolon of human trypanosoma cruzi infections. J. Trop. Med. Hyg.76, 175–179 (1973).
Narahashi, R., J. W. Moore, andW. R. Scott: Tetrodotoxin blockage of sodium contuctance increase in lobster giant axons. J. Gen. Physiol.47, 965–974 (1964).
Narahashi, R.: Mechanism of action of tetrodotoxin and sagitotoxin on excitable membranes. Fed. Proc.31, 1124–1132 (1972).
Palade, G. E., andS. L. Palay: Electron microscope observation of intraneuronal and neuromuscular synapses. Anat. Rec.118, 335 (1954).
Pellegrino de Iraldi, A., andDe Robertis, E.: The neurotubular system of the Axon and the Origin of Granulated and non-Granulated Vesicles in Regenerating Nerves. Z. Zeilforsch. Mikrosk. Anat.87, 330–344 (1968).
Reynolds, E. S.: The use of lead citrate at high pH as an electron opaque stain in electron microscopy. J. Cell Biol.17, 208–212 (1963).
Tafuri, W. L.: Ultraestruture of the vesicular component in the intramural nervous system of the guinea-pig's intestine. Naturforsch.795, 622–625 (1964).
Tafuri, W. L., T. A. Maria, J. E. H. Pittella, L. Bogliolo, W. Hial: purified scorpion toxin on vesicular components in the myenteric plexus on the rat. Toxicon9, 427 (1971).
Tafuri, W. L., T. A. Maria, L. Bogliolo, J. E. H. Pittella, W. Hial, andC. R. Diniz: Intramural nervous system of the colon and substance P in Hirschprung's disease. Virchows Arch. A Path. Anat. Histol.362, 41–50 (1974).
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Tafuri, W.L., Maria, T.A., Freire-Maia, L. et al. Effect of the scorpion toxin on the granular vesicles in the Auerbach's plexus of the rat ileum. J. Neural Transmission 35, 233–240 (1974). https://doi.org/10.1007/BF01258954
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DOI: https://doi.org/10.1007/BF01258954