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Influence of stress on the amount of “Gomori-positive” granules in the outer layer of the median eminence of bilaterally adrenalectomized rats

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Summary

The effect of an experimentally induced stress reaction on the amount of “Gomori-positive” granules, demonstrable in the median eminence of bilaterally adrenalectomized rats was studied. Application of formalin s.c. or histamine i.m. from the 15th to the 17th day p.o., or i.p. injection of Pyrifer® from the 15th to 21st day p.o., with simultaneous administration of 1% NaCl drinking fluid, has no effect on the amount of granules. On the other hand, the amount of granules diminishes if the animals, during a 3-day treatment with formalin, histamine or Pyrifer®, are given solely tap water to drink.

Water deprivation from the 15th to the 17th day after bilateral adrenalectomy also causes a marked reduction in the amount of granules.

It is assumed that the granules represent the morphological correlate of the corticotropin-releasing factor (CRF). The findings are related to the results of pharmacological studies on the behaviour of the CRF-activity during stress.

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References

  • Akmayev, I. G., O. V. Fidelina, Z. A. Kabolova, A. P. Popov, andT. Schitkova: Morphological aspects of the hypothalamic-hypophyseal system. IV. Medial basal hypothalamus. An experimental morphological study. Z. Zellforsch.137, 493–512 (1973).

    PubMed  Google Scholar 

  • Anderson, E.: Adrenocorticotrophin-releasing hormone in peripheral blood: increase during stress. Science152, 379–380 (1966).

    Google Scholar 

  • Arko, H., E. Kivalo, andU. K. Rinne: Hypothalamo-hypophysial neurosecretion after the extirpation of various endocrine glands. Acta endocr. (Kbh)42, 293–299 (1963).

    Google Scholar 

  • Arimura, A., T. Saito, andA. V. Schally: Assays for corticotropin-releasing factor (CRF) using rats treated with morphine, chloropromazine, dexamethasone and nembutal. Endocrinology81, 235–245 (1967).

    PubMed  Google Scholar 

  • Bach, J. H., andK. H. Hennes: Einfluß von Hydrocortison auf die Menge „Gomori-positiver“ Substanzen in der Zona externa infundibuli bilateral adrenalektomierter Ratten. J. Neural Transmission33, 11–22 (1972).

    Google Scholar 

  • Bachmann, R.: Die Nebenniere. In: Handbuch der Mikroskopischen Anatomie des Menschen (Begr. vonW. von Möllendorff, fortgef. vonW. Bargmann), Bd. VI, Teil5. Berlin-Göttingen-Heidelberg: Springer. 1954.

    Google Scholar 

  • Bock, R.: Lichtmikroskopische Untersuchungen zur Frage eines morphologischen Äquivalents des Corticotropin-releasing factor. In: Aspects of Neuroendocrinology (Bargmann, W., andB. Scharrer, eds.), pp. 229 to 231. Berlin-Heidelberg-New York: Springer. 1970.

    Google Scholar 

  • Bock, R.: Morphometrische Untersuchungen zum histologischen Nachweis des Corticotropin-releasing factor im Infundibulum der Ratte. Z. Anat. Entwickl.-Gesch.137, 1–29 (1972).

    Google Scholar 

  • Bock, R., andR. von Forstner: Beiträge zur funktionellen Morphologie der Neurohypophyse. II. Vergleichsuntersuchungen histologischer Veränderungen im Infundibulum der Ratte nach beidseitiger Adrenalektomie und nach Hypophysektomie. Z. Zellforsch.94, 434–440 (1969).

    PubMed  Google Scholar 

  • Bock, R., R. von Forstner, K. aus der Mühlen, andPh.A. Stöhr: Beiträge zur funktioneilen Morphologie der Neurohypophyse. III. Über die Wirkung einer Corticoid- und ACTH-Behandlung auf das Auftreten „gomoripositiver“ Granula in der zona externa infundibuli von Ratten und Mäusen nach beidseitiger Adrenalektomie oder Hypophysektomie. Z. Zellforsch.96, 142–150 (1969).

    PubMed  Google Scholar 

  • Bock, R., andR. von Forstner: Beiträge zur funktioneilen Morphologie der Neurohypophyse. I. Über eine „gomoripositive“ Substanz in der zona externa infundibuli beidseitig adrenalektomierter weißer Mäuse. Z. Zellforsch.92, 130–148 (1968).

    PubMed  Google Scholar 

  • Brinkmann, H., andR. Bock: Quantitative Veränderungen „Gomoripositiver“ Substanzen in Infundibulum und Hypophysenhinterlappen der Ratte nach Adrenalektomie und Kochsalz-oder Durstbelastung. J. Neuro-Visceral Relat.32, 48–64 (1970).

    Google Scholar 

  • Brinkmann, H., andR. Bock: Influence of various corticoids on the augmentation of “Gomori-positive” granules in the median eminence of the rat following adrenalectomy. Naunyn-Schmiedeberg's Arch. Pharmacol.1973, 49–62.

  • Chan, C. T., D. de Wied, andM. Saffran: Comparison of assays for corticotropin-releasing activity. Endocrinology84, 967–972 (1969).

    PubMed  Google Scholar 

  • Clark, W. G., andR. H. Barnes: Effects of salts and adrenal cortical extracts upon toxicity of drugs. Proc. Soc. exper. Biol. Med.44, 340–344 (1940).

    Google Scholar 

  • Danford, H. G., andR. C. Herrin: Some physiological effects on salt restriction in the rat. Am. J. Physiol.165, 128–134 (1951).

    PubMed  Google Scholar 

  • De Groot, J., andG. W. Harris: Hypothalamic control of the anterior pituitary gland and blood lymphocyts. J. Physiol.111, 335–346 (1950).

    Google Scholar 

  • Dhariwal, A. P. S., S. M. Russel, S. M. McCann, andF. E. Yates: Assay of corticotropin-releasing factors by injection into the anterior pituitary of intact rats. Endocrinology84, 544–556 (1969).

    PubMed  Google Scholar 

  • Feldmann, M.: Wirkung von DOCA und Dexamethason auf die Menge „Gomori-positiver“ Granula in der Zona externa infundibuli bilateral adrenalektomierter Ratten. (In Vorbereitung.)

  • Fortier, C.: Nervous control of ACTH secretion. In: The pituitary gland (Harris, G. W., andB. T. Donovan, eds.), Vol. 2, pp. 195–234. London: Butterworths. 1966.

    Google Scholar 

  • Fortier, C., andH. Selye: Adrenocorticotrophic effect of stress after severance of the hypothalamo-hypophyseal pathways. Am. J. Physiol,159, 433–439 (1949).

    PubMed  Google Scholar 

  • Gomori, G.: Observations with differential stains on human islets of Langerhans. Amer. J. Path.17, 395–406 (1941).

    Google Scholar 

  • Gruber, K., andE. M. Oberndörfer: Hypophysen-Nebennierenrindensystem und Leukozytenregulation, studiert am Modell des Pyriferversuches. Ärztl. Forschung8, I/523-I/527 (1954).

    Google Scholar 

  • Guillemin, R.: The adenohypophysis and its hypothalamic control. Ann. Rev. Physiol.29, 313–348 (1967).

    Google Scholar 

  • Halász, B., M. A. Slusher, andR. A. Gorski: Adrenocorticotrophic hormone secretion in rats after partial or total deafferentation of the medial basal hypothalamus. Neuroendocrinology2, 43–55 (1967).

    Google Scholar 

  • Helwig, B.: Moderne Arzneimittel. Eine Spezialitätenkunde nach Indikationsgebieten für Ärzte und Apotheker, 2nd ed., p. 1059. Stuttgart: Wissenschaftliche Verlagsgesellschaft m. b. H. 1961.

    Google Scholar 

  • Hiroshige, T., T. Sato, andK. Abe: Dynamic changes in the hypothalamic content of the corticotropin-releasing factor following noxious stimuli: delayed response in early neonates in comparison with biphasic response in adult rats. Endocrinology89, 1287–1294 (1971).

    PubMed  Google Scholar 

  • Hodges, J. R., andM. T. Jones: Changes in the pituitary corticotrophic function in the adrenalectomized rat. J. Physiol.173, 190–200 (1964).

    PubMed  Google Scholar 

  • Hodges, J. R., andJ. Vernikos: Circulating corticotrophin in normal and adrenalectomized rats after stress. Acta Endocr.30, 188–196 (1959).

    PubMed  Google Scholar 

  • Hodges, J. R., andJ. Vernikos: The effects of hydrocortison on the level of corticotrophin in the blood and pituitary glands of adrenalectomized and of stressed adrenalectomized rats. J. Physiol.150, 683–693 (1960).

    PubMed  Google Scholar 

  • Ishii, S.: Classification and identification of neurosecretory granules in the median eminence. In: Brain-endocrine Interaction. Median Eminence: Structure and Function, Int. Symp. Munich 1971, pp. 119–141. Basel: Karger. 1972.

    Google Scholar 

  • Jacobowitz, D., B. H. Marks, andJ. Vernikos-Danellis: Effect of acute stress on the pituitary gland: Uptake of serine-1-C14 into ACTH. Endocrinology72, 592–597 (1963).

    PubMed  Google Scholar 

  • Kohn, H. J.: Changes in composition of blood plasma of the rat during acute radiation syndrome, and their partial mitigation by dibenamine and cortin. Am. J. Physiol.165, 27–42 (1951 a).

    PubMed  Google Scholar 

  • Kohn, H. J.: Effect of unmaturity, hypophysectomy and adrenalectomy upon changes in plasma of rat during acute radiation syndrome. Am. J. Physiol.165, 43–56 (1951 b).

    PubMed  Google Scholar 

  • McCann, S. M., andJ. C. Porter: Hypothalamic pituitary stimulating and inhibiting hormones. Phys. Rev.49, 240–284 (1969).

    Google Scholar 

  • Makara, G. B., E. Stark, andM. Palkovits: Afferent pathways of stressful stimuli: corticotrophin release after hypothalamic deafferentation. J. Endocrin.47, 411–416 (1970).

    Google Scholar 

  • Makara, G. B., E. Stark, M. Palkovits, T. Révész, andK. Mihaly: Afferent pathways of stressful stimuli: corticotrophin release after partial deafferentation of the medial basal hypothalamus. J. Endocrin.44, 187 to 193 (1969).

    Google Scholar 

  • Marks, B. H., andJ. Vernikos-Danellis: Effect of acute stress on the pituitary gland: Action of ethionine on stress-induced ACTH release. Endocrinology72, 582–587 (1963).

    Google Scholar 

  • Parry, H. B., andB. G. Livett: A new hypothalamic pathway to the median eminence containing neurophysin and its hypertrophy in sheep with natural scrapie. Nature242, 63–65 (1973).

    PubMed  Google Scholar 

  • Perla, D.: Effect of salt on resistance to histamine in rats. Proc. Soc. exp. Biol. Med.41, 234–235 (1939).

    Google Scholar 

  • Perla, D., D. G. Freimann, M. Sandberg, andS. S. Greenberg: Prevention of histamine and surgical shock by cortical hormone (desoxycorticosterone acetate and cortin) and saline. Proc. Soc. exp. Biol. Med.43, 397–404 (1940).

    Google Scholar 

  • Perla, D., andM. Sandberg: Effect of electrolyte disturbance on resistance to histamine poisoning in rats. Proc. Soc. exp. Bio. Med.41, 275–277 (1939).

    Google Scholar 

  • Pfeffer, K. H., andH. J. Staudinger: Über die Ausscheidung von Corticoiden im Urin unter normalen und pathologischen Bedingungen. Klin. Wschr.30, 257–264 (1952).

    PubMed  Google Scholar 

  • Porter, R. W.: Hypothalamic involvement in the pituitary adrenocortical response to stress stimuli. Am. J. Physiol.172, 515–519 (1953).

    PubMed  Google Scholar 

  • Ravin, H. A., J. R. Demon, andH. Jensen: Electrolyte shifts and electrocardiographie changes during Tourniquet shock in rats. Am. J. Physiol.178, 419–426 (1954).

    PubMed  Google Scholar 

  • Rinne, U. K.: Experimental electron microscopic studies on the neurovascular link between the hypothalamus and anterior pituitary. In: Aspects of Neuroendocrinology (Bargmann, W., andB. Scharrer, eds.), pp. 220–228. Berlin-Heidelberg-New York: Springer. 1970.

    Google Scholar 

  • Rinne, U. K.: Effect of adrenalectomy on the ultrastructure and catecholamine fluorescence of the nerve endings in the median eminence of the rat. In: Brain-Endocrine Interaction. Median Eminence: Structure and Function, Int. Symp. Munich 1971, pp. 164–170. Basel: Karger. 1972.

    Google Scholar 

  • Rivier, C., W. Vale, andR. Guillemin: An in vivo corticotropin releasing factor (CRF) assay based on plasma levels of radioimmunoassayable ACTH. Proc. Soc. exp. Biol. Med.142, 842–845 (1973).

    PubMed  Google Scholar 

  • Sayers, G., andM. A. Sayers: Regulation of the pituitary adrenocorticotrophic activity during the response of the rat to acute stress. Endocrinology40, 265–273 (1947).

    Google Scholar 

  • Schneider, E., A. Blömer, R. Bock, H. Brinkmann, andH. G. Goslar: Verhalten „Gomori-positiver“ Granula im Infundibulum verschiedener Säugerspezies nach Adrenalektomie. Zugleich ein Beitrag zur speziesdifferenten Enzymausstattung von Neurohypophyse und Ependym des III. Ventrikels. Acta histochem.48, 172–190 (1974).

    Google Scholar 

  • Selye, H.: A syndrome produced by diverse nocuous agents. Nature138, 32 (1936).

    Google Scholar 

  • Selye, H.: Studies on adaptation. Endocrinology21, 169–188 (1937).

    Google Scholar 

  • Selye, H.: The general adaptation syndrome and the disease of adaptation. J. clin. Endocr.6, 117–230 (1946).

    Google Scholar 

  • Selye, H.: Stress (The physiology and pathology of exposure to systemic stress). Montreal: Acta Med. Publ. 1950.

    Google Scholar 

  • Selye, H.: Annual Report on Stress. Montreal: Acta Inc. Med. Publ. 1951.

    Google Scholar 

  • Selye, H.: Einführung in die Lehre vom Adaptationssyndrom. Stuttgart: G. Thieme. 1953.

    Google Scholar 

  • Smelik, P. G.: Relation between blood level of corticoids and their inhibiting effect on the hypophyseal stress response. Proc. Soc. exp. Biol. Med.113, 616–619 (1963).

    PubMed  Google Scholar 

  • Stöbr, Ph. A.: Über quantitative Veränderungen „gomori-positiver“ Substanzen in Infundibulum und Hypophysenhinterlappen der Ratte nach beidseitiger Adrenalektomie. Z. Zellforsch.94, 425–433 (1969).

    PubMed  Google Scholar 

  • Sydnor, K. L., andG. Sayers: Blood and pituitary ACTH in intact and adrenalectomized rats after stress. Endocrinology55, 621–631 (1954).

    PubMed  Google Scholar 

  • Vernikos-Danellis, J.: Effect of acute stress on the pituitary gland: changes in blood and pituitary ACTH concentrations. Endocrinology72, 574 to 581 (1963).

    Google Scholar 

  • Vernikos-Danellis, J.: Estimation of corticotropin-releasing activity of rat hypothalamus and neurohypophysis before and after stress. Endocrinology75, 514–520 (1964).

    PubMed  Google Scholar 

  • Vernikos-Danellis, J.: Effect of stress, adrenalectomy, hypophysectomy and hydrocortisone on the corticotropin-releasing activity of rat median eminence. Endocrinology76, 122–126 (1965 a).

    PubMed  Google Scholar 

  • Vernikos-Danellis, J.: Regulation of the synthesis and release of ACTH. Vitamines and Hormones23, 97–152 (1965 b).

    Google Scholar 

  • White, J. G.: Blood changes during the adaptation syndrome in sheep. J. Endocr.7, 143–153 (1951).

    PubMed  Google Scholar 

  • Wittkowski, W., andR. Bock: Electron microscopical studies of the median eminence following interference with the feedback system anterior pituitary-adrenal cortex. In: Brain-Endocrine Interaction. Median Eminence: Structure and Function, Int. Symp. Munich 1971, pp. 171 to 180. Basel: Karger. 1972.

    Google Scholar 

  • Wittkowski, W., R. Bock, andCh. Franken: Elektronenmikroskopische Untersuchungen lichtmikroskopisch „Gomori-positiver“ Granula in Infundibulum und Hypophysenhinterlappen bilateral adrenalektomierter Ratten. In: Aspects of Neuroendocrinology (Bargmann, W., andB. Scharrer, eds.), pp. 324–328. Berlin-Heidelberg-New York: Springer. 1970.

    Google Scholar 

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  1. P. E. Schwabedal

    Present address: Anatomisches Institut der Universität Bonn, Federal Republic of Germany

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    Supported by grants from the “Landesamt für Forschung” NRW, Federal Republic of Germany.

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    Schwabedal, P.E. Influence of stress on the amount of “Gomori-positive” granules in the outer layer of the median eminence of bilaterally adrenalectomized rats. J. Neural Transmission 35, 217–231 (1974). https://doi.org/10.1007/BF01258953

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