Summary
Cerebrospinal fluid 5-hydroxyindoleacetic acid (5-HIAA), homovanillic acid (HVA) and tryptophan (TRY) were measured in 36 female patients with alcohol dependence defined by strict operational criteria. Results were compared with a control group of 32 women hospitalized in a neurological unit for peripheral diseases. Several disorders presumably interfering with monoamine metabolism were carefully excluded and diet, psychomotor activity, diurnal variation, nutritional state and similar nonspecific variables controlled in both groups.
Only 5-HIAA was found to be decreased in dependent patients as compared to controls. Since 5-HIAA-but neither HVA nor TRY-correlated with body height in both groups, we eliminated its effect by analysis of covariance: as a result 5-HIAA difference became statistically significant but the other two biochemical measures remained unchanged.
A multivariate regression analysis with five independent clinical variables (body height, age, weight, years of alcohol abuse and days of abstinence) yielded significant determination coefficient R2 only for 5-HIAA. Regarding its components only body height and days of abstinence proved to be significantly correlated with 5-HIAA. Severity of withdrawal symptoms assessed on a rating scale in 14 patients with 4 days or less of abstinence correlated marginally with HVA but not with 5-HIAA. This latter metabolite decreased only in patients with 5 days or more of abstinence; the difference between 5-HIAA levels of short-term and longterm abstinence subgroups was statistically highly significant.
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References
Aghajanian, G. K., Wang, R. Y. Physiology and pharmacology of central serotonergic neurons. In: Psychopharmacology, a Generation of Progress (Lipton, M. A., DiMascio, A., Killam, K. F., eds.), pp. 171–183. New York: Raven Press. 1978.
Ahtee, L., Eriksson, K. Regional distribution of brain serotonin in rat strains selected for their alcohol intake. Ann. N.Y. Acad. Sci.215, 126–134 (1973).
Aizenstein, M. L., Korf, J. On the elimination of centrally formed 5-HIAA by cerebrospinal fluid and urine. J. Neurochem.32, 1227–1233 (1979).
Åsberg, M., Bertilsson, L. Serotonin in depressive illness, studies of CSF 5-HIAA. In: Neuropsychopharmacology (Saletu, B., Berner, P., Hollister, L. E., eds.), pp. 105–115. Oxford: Pergamon Press. 1979.
Badawy, A. A., Evans, M. Alcohol and tryptophan metabolism. J. Alcohol.9, 97–116 (1974).
Ballenger, J. C., Goodwin, F. K., Major, L. F., Brown, G. L. Alcohol and central serotonin metabolism in man. Arch. Gen. Psychiat.36, 224–227 (1979).
Banki, C. M. 5-hydroxytryptamine content of the whole blood in psychiatric illness and alcoholism. Acta psychiat. Scand.57, 232–238 (1978).
Berger, P. A., Faull, K. F., Kilkowski, J. Cerebrospinal fluid monoamine metabolites in depression and schizophrenia. Amer. J. Psychiat.137, 174–180 (1980).
Cochran, E., Robins, E., Grote, S. Regional serotonin levels in brain, a comparison of depressive and alcoholic suicides with controls. Biol. Psychiat.11, 283–294 (1976).
Curzon, G., Marsden, C. A. Metabolism of a tryptophan load in the hypothalamus and other brain regions. J. Neurochem.25, 251–256 (1975).
Curzon, G. Transmitter amines in brain disease. In: Biochemistry and Neurological Disease (Davison, A. N., ed.). Oxford: Blackwell. 1976.
Curzon, G., Kantamaneni, B. D., van Boxel, P. Substances related to serotonin in plasma and in lumbar and ventricular CSF of psychiatric patients. Acta psychiat. Scand.61, suppl. 280, 3–20 (1980).
Denckla, W. D., Dewey, H. K. The determination of tryptophan in plasma, liver and urine. J. Lab. Clin. Med.69, 160–169 (1967).
Ellingboe, J. Effect of alcohol on neurochemical processes. In: Psychopharmacology, a Generation of Progress (Lipton, M. A., DiMascio, A., Killam, K. F., eds.), pp. 1653–1664. New York: Raven Press. 1978.
Gottfries, C. G., Oreland, L., Wiberg, A., Winblad, B. Lowered MAO activity in brain from alcoholic suicides. J. Neurochem.25, 667–673 (1975).
Keeler, M. H., Taylor, C. I., Miller, W. C. Are all recently detoxified alcoholics depressed? Amer. J. Psychiat.136, 586–588 (1979).
Kemerer, V. F., Lichtenfeld, K. M., Koch, T. R. A column Chromatographie method for the determination of 5-HT (serotonin) and 5-HIAA in cerebrospinal fluid. Clin. Chim. Acta92, 81–85 (1979).
Knell, A. J., Davidson, A. R., Willians, R. Dopamine and serotonin metabolism in hepatic encephalopathy. Brit. med. J.1, 549–551 (1974).
Körösi, J. Studies with 5 H-2, 3-benzodiazepines. Ther. Hung.23, 4–8 (1975).
Mena, M. A., Herrero, E. Monoamine metabolism in rat brain regions following long-term alcohol treatment. J. Neural Transm.47, 227–236 (1980).
Oreland, L. MAO activity and affective illness. Acta psychiat. Scand.61, suppl. 280, 41–47 (1980).
Pickett, R. A., Collins, A. C. Use of genetic analysis to test the potential role of serotonin in alcohol preference. Life Sci.17, 1291–1296 (1975).
van der Poel, F. W., van Praag, H. M., Korf, J. Evidence for a probenecidsensitive transport system of acid monoamine metabolites from the spinal subarachnoid space. Psychopharmacol.52, 35–40 (1977).
Post, R. M., Goodwin, F. K. Studies of CSF amine metabolites in depressed patients, conceptual problems and theoretical implications. In: The Psychobiology of Depression (Mendels, J., ed.), pp. 47–67. New York: Spectrum Publ. 1975.
van Praag, H. M. Depression and Schizophrenia, p. 81. New York: Spectrum Publ. 1977.
van Praag, H. M. Central monoamine metabolism in depression. Comprehens. Psychiat.21, 30–54 (1980).
Reis, J. A possible role of central noradrenergic neurons in withdrawal states from alcohol. Ann. N.Y. Acad. Sci.215, 249–252 (1973).
Rolf, R. H., Matz, D. R., Brune, G. G. Serotoninstoffwechsel bei chronischen Alkoholikern. Experientia34, 74–75 (1978).
Spitzer, R. L., Williams, J. B. W., Skodol, A. E. DSM-III. The major achievements and an overview. Amer. J. Psychiat.137, 151–164 (1980).
Stahl, S. M. The human platelet. Arch. Gen. Psychiat.34, 509–516 (1977).
Takahashi, S., Yamane, H., Kondo, H., Tani, N. CSF monoamine metabolites in alcoholism, a comparative study with depression. Pol. Psychiat. Neurol. Jap.28, 347–354 (1974).
Takahashi, S., Tani, N., Yamane, H. MAO activity in blood platelets in alcoholics. Fol. Psychiat. Neurol. Jap.30, 455–462 (1976).
Truitt, E. B. A biogenic amine hypothesis for alcohol tolerance. Ann. N.Y. Acad. Sci.215, 177–182 (1973).
Weissman, M. M., Myers, J. K. Clinical depression in alcoholism. Amer. J. Psychiat.137, 372–373 (1980).
Westerink, B. H. C., Korf, J. Determination of nanogram amounts of HVA in the central nervous system with a rapid semiautomated fluorometric method. Biochem. Med.12, 106–115 (1975).
Wiberg, A., Wahlström, G., Oreland, L. Brain monoamine oxidase activity after chronic ethanol treatment of rats. Psychopharmacol.52, 111–113 (1977).
Young, S. N., Lal, S., Sourkes, S. Relationship between tryptophan in serum, CSF, and 5-HIAA in CSF of man, effect of cirrhosis of liver and probenecid. J. Neurol. Neurosurg. Psychiat.38, 322–331 (1975).
Young, S. N., Etienne, P., Sourkes, S. Relationship between rat brain and cisternal CSF tryptophan concentration. J. Neurol. Neurosurg. Psychiat.39, 233–243 (1976).
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Banki, C.M. Factors influencing monoamine metabolites and tryptophan in patients with alcohol dependence. J. Neural Transmission 50, 89–101 (1981). https://doi.org/10.1007/BF01249132
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DOI: https://doi.org/10.1007/BF01249132