Summary
The present study was undertaken with the aim of evaluating the sensitivity and convenience of a stress model reflecting sympatho-adrenal activation. Rats of both sexes were subjected to mild stress, consisting of four body temperature recordings, and investigated with regard to adrenal and heart catecholamine levels. Adrenal dopamine (DA) levels were considered to reflect medullary synthesis activity and heart adrenaline (A) concentrations were presumed to reflect A released from the adrenals. Expressed in relation to heart weight, adrenal catecholamine levels were about 50% higher in female rats. Following stress, adrenal DA levels were enhanced in both male and female rats; the magnitude of the stress response appeared similar in the two sexes. Concentrations of A in the hearts of unstressed animals were about 85% higher in females than in males but rose to a similar extent in male and female rats following stress. On the other hand, the stress-induced alterations in heart DA and NA concentrations, reflecting sympathetic activity, were gender-dependent.
The advantages of the present model in relation to other techniques for measuring sympatho-adrenal activation in response to mild stress are discussed.
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References
Anton AH, Sayre DF (1962) A study of the factors affecting the aluminium oxide-trihydroxyindole procedure for the analysis of catecholamines. J Pharmacol Exp Ther 138: 360–375
Bliss EL, Ailion J, Zwanziger J (1968) Metabolism of norepinephrine, serotonin and dopamine in rat brain with stress. J Pharmacol Exp Ther 164: 122–134
Bliss EL, Ailion J (1971) Relationship of stress and activity to brain dopamine and homovanillic acid. Life Sci 10: 1161–1169
Brown RM, Snider SR, Carlsson A (1974) Changes in biogenic amine synthesis and turnover induced by hypoxia and/or foot shock stress. II. The central nervous system. J Neural Transm 35: 293–305
Carlsson A, Snider SR, Almgren O, Lindqvist M (1973) The neurogenic short-term control of catecholamine synthesis and release in the sympatho-adrenal system, as reflected in the levels of endogenous dopamine and β-hydroxylated catecholamines. In: Usdin E, Snyder SH (eds) Frontiers in catecholamine research. Pergamon Press, Oxford, pp 551–556
Collins A (1985) Sex differences in psychoneuroendocrine stress responses. Thesis, University of Stockholm, Stockholm, Sweden. ISBN 91-7146-443-3
Crawley JN, Maas JW, Roth RH (1980) Biochemical evidence for simultaneous activation of multiple locus coeruleus efferents. Life Sci 26: 1373–1378
Curzon G, Green AR (1969) Effects of immobilization on rat liver tryptophan pyrrolase and brain 5-hydroxytryptamine metabolism. Br J Pharmacol 37: 689–697
Curzon G, Knott PJ (1974) Effects on plasma and brain tryptophan in the rat of drugs and hormones that influence the concentration of unesterified fatty acid in the plasma. Br J Pharmacol 50: 197–204
Curzon G, Joseph MH, Knott PJ (1972) Effects of immobilization and food deprivation on rat brain tryptophan metabolism. J Neurochem 19: 1967–1974
Demarest KT, Moore KE, Riegle GD (1985) Acute restraint stress decreases tuberoinfundibular dopaminergic neuronal activity: evidence for a differential response in male versus female rats. Neuroendocrinology 41: 504–510
Eriksson T, Carlsson A (1982) Isoprenaline increases brain concentrations of administered L-dopa and L-tryptophan in the rat. Psychopharmacology 77: 98–100
Euler US (1956) Noradrenaline. Chemistry, physiology, pharmacology and clinical aspects. ChC Thomas, Springfield, Illinois
Fadda F, Argiolas A, Melis MR, Tissari AH, Onali PL, Gessa GL (1978) Stress-induced increase in 3,4-dihydroxyphenylacetic acid (DOPAC) levels in the cerebral cortex and in n accumbens: reversal by diazepam. Life Sci 23: 2219–2224
Franklin KBJ, Kelly SJ (1986) Sympathetic control of tryptophan uptake and morphine analgesia in stressed rats. Eur J Pharmacol 126: 145–150
Frantz AG (1978) Physiology in medicine. Prolactin. N Engl J Med 298: 201–207
Guillemin R, Vargo T, Rossier J, Minick S, Ling N, Rivier C, Vale W, Bloom F (1977) β-Endorphin and adrenocorticotropin are secreted concomitantly by the pituitary gland. Science 197: 1367–1368
Joseph MH, Kennett GA (1983) Stress-induced release of 5-HT in the hippocampus and its dependence on increased tryptophan availability: an in vivo electrochemical study. Brain Res 270: 251–257
Kant GJ, Lenox RH, Bunnell BN, Mougey EH, Pennington LL, Meyerhoff JL (1983a) Comparison of stress response in male and female rats: pituitary cyclic AMP and plasma prolactin, growth hormone and corticosterone. Psychoneuroendocrinology 8: 421–428
Kant GJ, Mougey EH, Pennington LL, Meyerhoff JL (1983b) Graded footshock stress elevates pituitary cyclic AMP and plasma β-endorphin, β-LPH, corticosterone and prolactin. Life Sci 33: 2657–2663
Kennett GA, Chaouloff F, Marcou M, Curzon G (1986) Female rats are more vulnerable than males in an animal model of depression: the possible role of serotonin. Brain Res 382: 416–421
Kitay JI (1961) Sex differences in adrenal cortical secretion in the rat. Endocrinology 68: 818–824
Korf J, Aghajanian GK, Roth RH (1973) Increased turnover of norepinephrine in the rat cerebral cortex during stress: role of the locus coeruleus. Neuropharmacology 12: 933–938
Krulich L, Hefco E, Illner P, Read CB (1974) The effects of acute stress on the secretion of LH, FSH, prolactin and GH in the normal male rat, with comments on their statistical evaluation. Neuroendocrinology 16: 293–311
Kurokawa N, Suematsu H, Tamai H, Esaki M, Aoki H, Ikemi Y (1977) Effect of emotional stress on human growth hormone secretion. J Psychosom Res 21: 231–235
Kvetnansky R, Weise VK, Kopin IJ (1970) Elevation of adrenal tyrosine hydroxylase and phenylethanolamine-N-methyl transferase by repeated immobilization of rats. Endocrinology 87: 744–749
Kvetnansky R, Sun CL, Lake CR, Thoa N, Torda T, Kopin IJ (1978) Effect of handling and forced immobilization on rat plasma levels of epinephrine, norepinephrine, and dopamine-β-hydroxylase. Endocrinology 103: 1868–1873
Livezey GT, Miller JM, Vogel WH (1985) Plasma norepinephrine, epinephrine and corticosterone stress responses to restraint in individual male and female rats, and their correlations. Neurosci Lett 62: 51–56
Mason JW (1971) A re-evaluation of the concept of “non-specificity” in stress theory. J Psychiatr Res 8: 323–333
Reinhard JF, Bannon MJ, Roth RH (1982) Acceleration by stress of dopamine synthesis and metabolism in prefrontal cortex: antagonism by diazepam. Naunyn Schmiedebergs Arch Pharmacol 318: 374–377
Seggie JA, Brown GM (1975) Stress response patterns of plasma corticosterone, prolactin, and growth hormone in the rat, following handling or exposure to novel environment. Can J Physiol Pharmacol 53: 629–637
Snider SR, Carlsson A (1972) The adrenal dopamine as an indicator of adrenomedullary hormone biosynthesis. Naunyn Schmiedebergs Arch Pharmacol 275: 347–357
Snider SR, Almgren O, Carlsson A (1973) The occurrence and functional significance of dopamine in some peripheral adrenergic nerves of the rat. Naunyn Schmiedebergs Arch Pharmacol 278: 1–12
Snider SR, Brown RM, Carlsson A (1974) Changes in biogenic amine synthesis and turnover induced by hypoxia and/or foot shock stress I. The adrenal medulla. J Neural Transm 35: 283–291
Svensson K (1986) Dopamine autoreceptor antagonists: a new class of central stimulants. Thesis, University of Göteborg, Göteborg, Sweden. ISBN 91-7900-078-9
Terry LC, Willoughby JO, Brazeau P, Martin JB (1976) Antiserum to somatostatin prevents stress-induced inhibition of growth hormone secretion in the rat. Science 192: 565–567
Thierry A-M, Javoy F, Glowinski J, Kety SS (1968) Effects of stress on the metabolism of norepinephrine, dopamine and serotonin in the central nervous system of the rat. I. Modifications of norepinephrine turnover. J Pharmacol Exp Ther 163: 163–171
Thierry A-M, Tassin JP, Blanc G, Glowinski J (1976) Selective activation of the mesocortical DA system by stress. Nature 263: 242–244
Thoenen H (1970) Induction of tyrosine hydroxylase in peripheral and central adrenergic neurones by cold-exposure of rats. Nature 228: 861–862
Tissari AH, Argiolas A, Fadda F, Serra G, Gessa GL (1979) Foot-shock stress accelerates non-striatal dopamine synthesis without activating tyrosine hydroxylase. Naunyn Schmiedebergs Arch Pharmacol 308: 155–157
Vigas M, Malatinsky J, Nemeth S, Jurcovicova J (1977) Alpha-adrenergic control of growth hormone release during surgical stress in man. Metabolism 26: 399–402
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Carlsson, M., Carlsson, A. Effects of mild stress on adrenal and heart catecholamines in male and female rats. J. Neural Transmission 77, 217–226 (1989). https://doi.org/10.1007/BF01248934
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DOI: https://doi.org/10.1007/BF01248934