Summary
Schwann cell proliferation is considered an essential part of Wallerian degeneration after nerve damage. Laminin, an important component of the extracellular matrix and produced by Schwann cells, provides a preferred substrate for outgrowing axons. To study whether low energy (He-Ne) laser irradiation may exert a positive effect on nerve regeneration through an effect on Schwann cells, its effect was evaluatedin vitro. Schwann cells were isolated from sciatic nerves of 4–5-day-old Wistar rates and cultured on 96-multiwell plates. The cells were irradiated by a He-Ne laser beam (632.8 nm, 5.98 mW) that was optically expanded to a beam width of 4 mm. During irradiation the plate was kept in an air-tight box equilibrated with humidified air containing 5% CO2 and kept at 37 ° C. At three consecutive days, starting either at day 5 or day 8, cells were irradiated each day for 0.5, 1, 2, 5 or 10 min. Both cell number and laminin production were determined for each irradiation condition (n=5) within one experiment. Schwann cells that were irradiated from day 8 on were hardly affected by laser irradiation. However, the proliferation of cells that were irradiated starting on day 5 was significantly increased after 1, 2, and 5 min of daily irradiation, compared to non-irradiated control cultures. The laminin production per cell of these Schwann cells was not significantly altered. From these results we conclude that He-Ne laser irradiation can modulate proliferation of rat Schwann cellsin vitro in a dose-dependent manner.
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Assia, E., Rosner, M., Belkin, M., Solomon, A. &Schwartz, M. (1989) Temporal parameters of low energy laser irradiation for optimal delay of post-traumatic degeneration of rat optic nerve.Brain Research 476, 205–12.
Balboni, G. C., Brandi, M. L., Zonefrati, R. &Repice, F. (1986) Effects of He-Ne/I.R. laser irradiation on two lines of normal human fibroblastsin vitro.Archivio Italiano di Anatomia e di Embriologia 91, 179–88.
Basford, J. R. (1989) The clinical and experimental status of low energy laser therapy.Critical Reviews in Physical and Rehabilitation Medicine 1, 1–9.
Basford, J. R., Daube, J. R., Hallman, H. O., Millard, T. L. &Moyer, S. K. (1990) Does low-intensity helium-neon laser irradiation alter sensory nerve action potentials or distal latencies?Lasers in Surgery and Medicine 10, 35–9.
Belkin, M. &Schwartz, M. (1989) New biological phenomena associated with laser radiation.Health Physics 56, 687–90.
Bosatra, M., Jucci, A., Olliaro, P., Quacci, D. &Sacchi, S. (1984)In vitro fibroblast and dermis fibroblast activation by laser irradiation at low energy.Dermatologica 168, 157–62.
Boulton, M. &Marshall, J. (1986) He-Ne stimulation of human fibroblast proliferation and attachmentin vitro.Lasers in the Life Sciences 1, 125–34.
Brockes, J. P., Fields, K. L. &Raff, M. C. (1979) Studies on cultured rat Schwann cells. I. Establishment of purified populations from cultures of peripheral nerve.Brain Research 165, 105–18.
Colver, G. B. (1989) Failure of a He-Ne laser to affect components of wound healingin vitro.British Journal of Dermatology 121, 179–86.
Fedoseyeva, G. E., Smolyaninova, N. K., Karu, T. I. &Zelenin, A. V. (1988) Human lymphocyte chromatin changes following irradiation with a He-Ne laser.Lasers in the Life Sciences 2, 197–205.
Friedman, H., Lubart, R., Laulicht, I. &Rochkind, S. (1991) A possible explanation of laser-induced stimulation and damage of cell cultures.Journal of Photochemistry and Photobiology, B: Biology 11, 87–95.
Glassberg, E., Lask, G. P., Tan, E. M. L. &Uitto, J. (1988) Cellular effects of the pulsed tunable dye laser at 577 nanometers on human endothelial cells, fibroblasts, and erythrocytes: anin vitro study.Lasers in Surgery and Medicine 8, 567–72.
Greathouse, D. G., Currier, D. P. &Gilmore, R. L. (1985) Effects of clinical infrared laser on superficial radial nerve conduction.Physical Therapy 65, 1184–7.
Hallman, H. O., Basford, J. R., O'Brien, J. F. &Cummins, L. A. (1988) Does low-energy helium-neon laser irradiation alter ‘in vitro’ replication of human fibroblasts?Lasers in Surgery and Medicine 8, 125–9.
Inuoe, K., Nishioka, J. &Hukuda, S. (1989) Altered lymphocyte proliferation by low dosage laser irradiation.Clinical and Experimental Rheumatology 7, 521–3.
Karu, T. I. (1989) Photobiology of low-power laser effects.Health Physics 56, 691–704.
Karu, T. I., Kalendo, G. S., Letokhov, V. S. &Lobko, V. V. (1984) Biostimulation of HeLa cells by low-intensity visible light II/III.Il Nuovo Cimento 3D, 309–25.
Karu, T. I., Tiphlova, O. A., Letokhov, V. S. &Lobko, V. V. (1983) Stimulation ofE. coli growth by laser and incoherent red light.Il Nuovo Cimento 2D, 1138–44.
King, P. R. (1989) Low level laser therapy: a review.Lasers in Medical Science 4, 141–50.
Kubasova, T., Kovacs, L., Somosy, Z., Unk, P. U. &Kokai, A. (1984) Biological effects of the HeNe laser: investigations on functional and micromorphological alterations of cell membranes,in vitro.Lasers in Surgery and Medicine 4, 381–8.
Lam, T. S., Abergel, R. P., Meeker, C. A., Castel, J. C., Dwyer, R. M. &Uitto, J. (1986) Laser stimulation of collagen synthesis in human skin fibroblast cultures.Lasers in the Life Sciences 1, 61–77.
Lipton, S. A. &Kater, S. B. (1989) Neurotransmitter regulation of neuronal outgrowth, plasticity and survival.Trends in Neurosciences 12, 265–70.
Manteifel, V. M., Andreichuk, T. N. &Karu, T. I. (1991) Reaction of the mitochondrial apparatus of the lymphocytes to irradition by a He-Ne laser and to the mitogen phytohemagglutinin.Molecular Biology 25, 229–35.
Meyers, A. D., Joyce, J. &Cohen, J. J. (1987) Effects of low-watt helium neon laser radiation on human lymphocyte cultures.Lasers in Surgery and Medicine 6, 540–2.
Naveh, N., Bar-Ilan, A., Rosner, M., Schwartz, M., Weissman, C. &Belkin, M. (1990) Low-energy laser irradiation — a new measure for suppression of arachidonic acid metabolism in the optic nerve.Journal of Neuroscience Research 26, 386–9.
Nissan, M., Rochkind, S., Razon, N. &Bartal, A. (1986) HeNe laser irradiation delivered transcutaneously: its effect on the sciatic nerve of rats.Lasers in Surgery and Medicine 6, 435–8.
Passarella, S., Casamassima, E., Quagliariello, E., Caretto, G. &Jirillo, E. (1985) Quantitative analysis of lymphocyte-salmonella interaction and effect of lymphocyte irradiation by helium-neon laser.Biochemical and Biophysical Research Communications 130, 546–52.
Pourreau-Schneider, N., Ahmed, A., Soudry, M., Jacquemier, J., Kopp, F., Franquin, J. C. &Martin, P. M. (1990) Helium-neon laser treatment transforms fibroblasts into myofibroblasts.American Journal of Pathology 137, 171–8.
Rochkind, S., Nissan, M., Razon, N., Schwartz, M. &Bartal, A. (1986) Electrophysiological effect of HeNe laser on normal and injured sciatic nerve in the rat.Acta Neurochirurgica 83, 125–30.
Rochkind, S., Nissan, M., Lubart, R., Avram, J. &Bartal, A. (1988) Thein-vivo-nerve response to direct low-energy-laser irradiation.Acta Neurochirurgica 94, 74–7.
Rochkind, S., Rousso, M., Nissan, M., Villarreal, M., Barr-Nea, L. &Rees, D. G. (1989) Systemic effects of low-power laser irradiation on the peripheral and central nervous system, cutaneous wounds, and burns.Lasers in Surgery and Medicine 9, 174–82.
Salet, C., Passarella, S. &Quagliariello, E. (1987) Review article: effects of selective irradiation on mammalian mitochondria.Photochemistry and Photobiology 45, 433–8.
Schwartz, M., Doron, H., Erlich, M., Lavie, V., Ben Bassat, J., Belkin, M. &Rochkind, S. (1987) Effects of low-energy He-Ne laser irradiation on posttraumatic degeneration of adult rabbit optic nerve.Lasers in Surgery and Medicine 7, 51–5.
Snyder-Mackler, L. &Bork, C. E. (1988) Effect of helium-neon laser irradiation on peripheral sensory nerve latency.Physical Therapy 68, 223–5.
Tiphlova, O. &Karu, T. (1991) Action of low-intensity laser radiation onEscherichia coli.CRC Critical Reviews in Biomedical Engineering 18, 387–412.
Van Breugel, H. H. F. I. &Bär, P. R. (1992) Power density and exposure time of He-Ne laser irradiation are more important than total energy dose in photo-biomodulation of human fibroblastsin vitro.Lasers in Surgery and Medicine 12, 528–37.
Van Der Neut, Sodaar, P. &Bär, P. R. (1988) An enzyme-linked immunoadsorbant assay for laminin,Neuroscience Research Communications 3, 119–25.
Walker, J. B. (1985) Temporary suppression of clonus in humans by brief photostimulation.Brain Research 340, 109–13.
Walker, J. B. &Akhanjee, L. K. (1985) Laser-induced somatosensory evoked potentials: evidence of photosensitivity in peripheral nerves.Brain Research 344, 281–5.
Wu, W., Ponnudurai, R., Katz, J., Pott, C. B., Chilcoat, R., Uncini, A., Rapport, S., Wade, P. &Mauro, A. (1987) Failure to confirm report of light-evoked response of peripheral nerve to low power helium-neon laser light stimulus.Brain Research 401, 407–8.
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Van Breugel, H.H.F.I., Bär, P.R. He-Ne laser irradiation affects proliferation of cultured rat Schwann cells in a dose-dependent manner. J Neurocytol 22, 185–190 (1993). https://doi.org/10.1007/BF01246357
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DOI: https://doi.org/10.1007/BF01246357