Abstract
The temporal course of epidermal basal cell proliferation in the wound of the pinna of rats was studied using bromodeoxyuridine (BrdU) immunohistochemistry. Following incisional wounding, the animals were sacrificed at intervals ranging from 0 hours to 32 days. Two biopsies were taken from each animal, one intravitally and one postmortem after 24 hours storage at 8°C. Specimens were incubated in a solution containing BrdU and embedded in paraffin. BrdU expression was demonstrated by a monoclonal antibody against BrdU. In both intravital and postmortem biopsies, the labelling indices increased significantly in the period from 32 to 60 hours post-injury. This suggests that DNS synthesis induced during life continues after death. Applied to forensic practice, the present findings point to the possibility of determining the vitality of a wound in postmortem tissue.
Zusammenfassung
Im Tierexperiment wurde die Zeitabhängigkeit der Proliferationsaktivität von epidermalen Basalzellen aus der Schnittwunde des Rattenohres unter Verwendung von BrdU (in vitro-Inkubation) durch Bestimmung des Markierungsindexes untersucht. Nach Schnittverletzung überlebten die Tiere unterschiedliche Zeitintervalle von 0 Stunden bis 32 Tage. Es erfolgten pro Tier je 2 Biopsieentnahmen: vital und 24 Stunden nach Eintritt des Todes (postmortal), währenddessen der Tierkörper bei 8°C im Kühlschrank gelagert wurde. Folgendes Ergebnis konnte gewonnen werden: Unter der Annahme eine p ≥ 0.01 nahm der Markierungsindex epidermaler Basalzellen nach 32 Stunden Überlebenszeiten bis zur 60. Stunde nach Wundsetzung signifikant zu. Diese Zunahme war nach vitaler Biopsie ebenso wie nach postmortaler Biopsie nachweisbar. Es ist somit davon auszugehen, daß nach der einmal erfolgten Proliferationsinduktion sich diese auch postmortal fortsetzt. Für die forensische Praxis ist festzustellen, daß auch an postmortalem Gewebe mindestens eine Vitalitätsbestimmung vorgenommen werden dürfte.
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Oehmichen, M., Cröpelin, A. Temporal course of intravital and postmortem proliferation of epidermal cells after mechanical injury. Int J Leg Med 107, 257–262 (1995). https://doi.org/10.1007/BF01245484
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DOI: https://doi.org/10.1007/BF01245484