Summary
After separation of aqueous extracts of sheep pineal bodies and sheep cerebral cortex on Sephadex G-25, a high fluorescence peak showing an excitation maximum at 305→310 nm and a fluorescence maximum at 350→355 nm was observed in pineal extracts, which could not be detected in cerebral cortex extracts. Separation of this pineal fluorescence peak on Sephadex G-10 with water elution, followed by gradient elution of water and NaOH, gave rise to several highly fluorescent peaks. From one of these it was possible to isolate a substance with Rf-values, using thinlayer chromatographic, fluorescence and mass spectra studies, identical with synthetic 5-hydroxyindole-3-acetic acid. From another peak a substance, identical with synthetic 5-methoxyindole-3-acetic acid, could be isolated. The results are compared with those obtained in previous studies.
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Zurburg, W., Ebels, I. Separation of pineal extracts by gelfiltration. J. Neural Transmission 36, 59–69 (1975). https://doi.org/10.1007/BF01243438
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DOI: https://doi.org/10.1007/BF01243438