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Neuropeptide Y enhances the inhibitory effects of clonidine on3H-noradrenaline release in synaptosomes isolated from the medulla oblongata of the male rat

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Summary

In studies on superfused synaptosomes from the rat medulla oblongata, the inhibitory effects of theα2-adrenergic agonist clonidine (0.1μM) on potassium (15 mM K+) induced3H-noradrenaline (NA) release was potentiated by 20%, when neuropeptide Y was added to the system. The effect of NPY was detectable at low concentrations (1 nM) and was not dose-dependent. Neuropeptide Y alone produced no significant effects on3H-NA release. The results may indicate the existence of a presynaptic NPY receptor on the noradrenaline and/or adrenaline nerve terminals, which may enhance the presynapticα2-adrenoreceptor function to inhibit3H-NA release.

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References

  • Agnati LF, Fuxe K, Benfenati F, Battistini N, Härfstrand A, Tatemoto K, Hökfelt T, Mutt V (1983 a) Neuropeptide Yin vitro selectively increases the number ofα2-adrenergic binding sites in membranes of the medulla oblongata of the rat. Acta Physiol Scand 118: 293–295

    Google Scholar 

  • Agnati LF, Fuxe K, Benfenati F, Zini I, Hökfelt T (1983 b) On the functional role of coexistence of 5-HT and substance P in bulbospinal 5-HT neurons. Substance P reduces affinity and increases density of3H-5-HT binding sites. Acta Physiol Scand 117: 299–301

    Google Scholar 

  • Agnati LF, Fuxe K, Locateili V, Benfenati F, Zini I, Panerai AE, El Etreby MF, Hökfelt T (1982) Neuroanatomical methods for the quantitative evaluation of coexistence of transmitters in nerve cells. Analysis of the ACTH- and betaendorphin immunoreactive nerve cell bodies of the mediobasal hypothalamus of the rat. J Neurosci Methods 5: 203–214

    Google Scholar 

  • Agnati LF, Fuxe K, Zoli M, Merlo Pich E, Benfenati F, Zini I, Goldstein M (1986) Aspects on the information handling by the central nervous system: Focus on cotransmission in the aged rat brain. Prog Brain Res (in press)

  • Allen YS, Adrian TE, Allen JM, Tatemoto K, Crow TJ, Bloom SR, Polak JM (1983) Neuropeptide Y distribution in the rat brain. Science 21: 877–879

    Google Scholar 

  • Allen JM, Adrian TE, Tatemoto K, Polak JM, Hughes J, Bloom SR (1982) Two novel related peptides, neuropeptide Y (NPY) and peptide YY (PYY) inhibit the contraction of the electrically-stimulated mouse vas deferens. Neuropeptides 3: 71–77

    Google Scholar 

  • Andén NE, Corrodi H, Fuxe K, Hökfelt B, Hökfelt T, Rydin C, Svensson T (1970) Evidence for a central noradrenaline receptor stimulation by clonidine. Life Sci 9/1: 513–523

    Google Scholar 

  • Andén NE, Grabowska, Strombom U (1976) Different alpha-adrenoceptors in central nervous system mediating biochemical and functional effects of clonidine and receptor blocking agents. Naunyn-Schmiedebergs' Arch Pharmacol 292: 43–52

    Google Scholar 

  • Cerrito F, Raiteri M (1979) Serotonin release in modulated by presynaptic autoreceptors. Eur J Pharmacol 57: 427–430

    Google Scholar 

  • Everitt BJ, Hökfelt T, Terenius L, Tatemoto K, Mutt V, Goldstein M (1984) Differential coexistence of neuropeptide Y (NPY-)like immunoreactivity with catecholamines in the central nervous system of the rat. Neuroscience 11/2: 443–462

    Google Scholar 

  • Fuxe K, Agnati LF (1985) Receptor-receptor interactions in the central nervous system. A new integrative mechanism in synapses. Med Res Rev 5: 441–482

    Google Scholar 

  • Fuxe K, Agnati LF, Benfenati F, Cimino M, Algeri S, Hökfelt T, Mutt V (1981) Modulation by cholecystokinins of3H-spiroperidol binding in rat striatum: Evidence for increased affinity and reduction of the number of binding sites. Acta Physiol Scand 113: 567–569

    Google Scholar 

  • Fuxe K, Agnati LF, Benfenati F, Celani M, Zini I, Zoli M, Mutt V (1983 a) Evidence for the existence of receptor-receptor interactions in the central nervous system. Studies on the regulation of monoamine receptors by neuropeptides. J Neural Transm [Suppl] 18: 165–179

    Google Scholar 

  • Fuxe K, Agnati LF, Härfstrand A, Jansson AM, Neumeyer A, Andersson K, Ruggeri M, Zoli M, Goldstein M (1986) Morphofunctional studies on the neuropeptide Y/adrenaline costoring nerve terminal systems in the dorsal cardiovascular region of the medulla oblongata. Focus on receptor-receptor interactions in cotransmission. Progr Brain Res (in press)

  • Fuxe K, Agnati LF, Härfstand A, Martire M, Goldstein M, Grimaldi R, Bernardi P, Zini I, Tatemoto K, Mutt V (1984) Evidence for a modulation by neuropeptide Y of the α2-adrenergic transmission line in central adrenaline synapses. New possibilities for treatment of hypertensivedisorders. Clin Exp Hypertens A 6: 1951–1956

    Google Scholar 

  • Fuxe K, Agnati LF, Härfstrand A, Zini I, Tatemoto K, Merlo Pich E, Hökfelt T, Mutt V, Terenius L (1983 b) Central administration of neuropeptide Y induces hypotension, bradypnea and EEG synchronization in the rat. Acta Physiol Scand 118: 189–192

    Google Scholar 

  • Fuxe K, Agnati LF, Zoli M, Härfstrand A, Grimaldi R, Bernardi P, Camurri M, Tucci F, Goldstein M (1985) Development of quantitative methods for the evaluation of the entity of coexistence of neuroactive substances in nerve terminal populations in discrete regulation of cotransmission. For Wenner-Gren Symposium on “Quantitative Neuroanatomy in Transmitter Research”, Stockholm, May 3–4. Macmillan, London, pp 157–174

    Google Scholar 

  • Fuxe K, Jonsson G, Bolme P, Andersson K, Agnati LF, Goldstein M, Hökfelt T (1979) Reduction of adrenaline turnover in cardiovascular areas of rat medulla oblongata by clonidine. Acta Physiol Scand 107: 177–179

    Google Scholar 

  • Gray EG, Whittaker VP (1962) The isolation of nerve endings from brain: an electron microscope study of cell fragments derived by homogenization and centrifugation. J Anat 96: 79–87

    Google Scholar 

  • Hollander and Wolfe (1973) Non-parametric statistical methods. John Wiley, New York

    Google Scholar 

  • Hökfelt T, Johansson O, Ljungdahl A, Lundberg JM, Schultzberg M (1980) Peptidergic neurons. Nature 284: 515–521

    Google Scholar 

  • Hökfelt T, Lundberg JM, Skirboll L, Johansson O, Schultzberg M, Vincent SR (1982) Coexistence of classical transmitters and peptides in neurons. In: Cotransmission. Macmillan, London, pp 77–126

    Google Scholar 

  • Härfstrand A, Fuxe K, Agnati LF, Ganten D, Eneroth P, Tatemoto K, Mutt V (1984) Studies on neuropeptide Y-catecholamine interactions in central cardiovascular regulation in theα-chloralose anaesthetized rat. Evidence for a possible new way of activating theα2-adrenergic transmission line. Clin Exp Hypertens A 6: 1947–1950

    Google Scholar 

  • Hunt SP, Emson PC, Gilbert R, Goldstein M, Kimmel JR (1981) Presence of avian pancreatic polypeptide-like immunoreactivity in catecholamine- and methionine enkephalin-containing neurons within the central nervous system. Neurosci Lett 21: 125–130

    Google Scholar 

  • Langer SZ, Briley M, Dubocovich ML (1980) Adrenergic receptor mechanisms in the central nervous system in relation to catecholamine neurons. In: Wenner-Gren (ed) Center international symposium series 33, pp 199–212

  • Langer SZ (1981) Presynaptic regulation of the release of catecholamines. Pharmacol Rev 33: 337–362

    Google Scholar 

  • Lundberg JM, Hedlund B, Änggård A, Fahrenkrug J, Hökfelt T, Tatemoto K, Bartfai T (1982 a) Costorage of peptides and classical transmitters in neurons. In: Systemic role of regulatory peptides. Schattauer, Stuttgart, pp 93–119

    Google Scholar 

  • Lundberg JM, Hökfelt T, Änggård A, Kimmel J, Goldstein M, Markey K (1980) Coexistence of an avian pancreatic polypeptide (APP) immunoreactive substance and catecholamines in some peripheral and central neurons. Acta Physiol Scand 110: 107–109

    Google Scholar 

  • Lundberg JM, Tatemoto K (1982) Pancreatic polypeptide family (APP, BPP, NPY and PYY) in relation to sympathetic vasoconstriction resistant to adrenoceptor blockade. Acta Physiol Scand 116: 393–402

    Google Scholar 

  • Lundberg JM, Terenius L, Hökfelt T, Martling CR, Tatemoto K, Mutt V, Polak J, Bloom S, Goldstein M (1982 b) Neuropeptide Y (NPY)-like immunoreactivity in peripheral noradrenergic neurons and effects of NPY on sympathetic function. Acta Physiol Scand 116: 477–480

    Google Scholar 

  • Mulder AH, Dismukes RK, De Langen CDJ, Werner J, Frankhuyzen AL (1980) Studies on presynapticα-adrenoceptors in the rat brain. Prog Pharmacol 3: 19–23

    Google Scholar 

  • Raiteri M, Angelini F, Levi G (1974) A simple apparatus for studying the release of neurotransmitters from synaptosomes. Eur J Pharmacol 25: 411–414

    Google Scholar 

  • Raiteri M, Levi G (1978) Release mechanisms for catecholamines and serotonin in synaptosomes. In: Reviews of neuroscience. Raven Press, New York, pp 77–130

    Google Scholar 

  • Smith JE, Lane JD, Shea PA, McBride WJ, Aprison MH (1975) A method for concurrent measurement of picomole quantities of acetylcholine, choline, dopamine, norapinephrine, serotonin, 5-hydroxytryptophan, 5-hydroxyindoleacetic acid, tryptophan, tyrosine, glycine, aspartate, glutamate, alanine and gamma-amino butyric acid in single tissue samples from different areas of rat central nervous system. Anal Biochem 64: 149–169

    Google Scholar 

  • Starke K (1981) Presynaptic receptors. Ann Rev Pharmacol Toxicol 21: 7–30

    Google Scholar 

  • Tatemoto K (1982) Neuropeptide Y. Complete aminoacid sequence of the brain peptide. Proc Natl Acad Sci USA 79: 5485–5489

    Google Scholar 

  • Tatemoto K, Carlquist M, Mutt V (1982) Neuropeptide Y-a novel brain peptide with structural similarities for peptide YY and pancreatic polypeptide. Nature 296: 659–660

    Google Scholar 

  • Varndell IM, Polak JM, Allen JM, Terenghi G, Bloom SR (1984) Neuropeptide tyrosine (NPY) immunoreactivity in norepinephrine-containing cells and nerves of the mammalian adrenal gland. Endocrinol 114/4: 1460–1462

    Google Scholar 

  • Zini I, Merlo Pich E, Fuxe K, Lenzi PL, Agnati LF, Härfstrand A, Mutt V, Tatemoto K, Moscara M (1984) Actions of centrally administered neuropeptide Y on EEG activity in different rat strains and in different phases of their circadian cycle. Acta Physiol Scand 122: 71–77

    Google Scholar 

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Author notes

  1. K. Fuxe

    Present address: Department of Histology, Karolinska Institute, Stockholm, Sweden

Authors and Affiliations

  1. Department of Pharmacology, Catholic University, Rome, Italy

    M. Martire, G. Pistritto & P. Preziosi

  2. Department of Human Physiology, University of Modena, Modena, Italy

    L. F. Agnati

Authors
  1. M. Martire
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  2. K. Fuxe
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  3. G. Pistritto
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  4. P. Preziosi
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  5. L. F. Agnati
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Martire, M., Fuxe, K., Pistritto, G. et al. Neuropeptide Y enhances the inhibitory effects of clonidine on3H-noradrenaline release in synaptosomes isolated from the medulla oblongata of the male rat. J. Neural Transmission 67, 113–124 (1986). https://doi.org/10.1007/BF01243364

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  • DOI: https://doi.org/10.1007/BF01243364

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