Summary
Efficiency of adenovirus transformation in hamster embryo cells is shown to depend on the careful control of cell density, virus adsorption under hypertonic conditions, and the ratio of cell transforming to cell killing virus.
Similar content being viewed by others
References
Rafajko, R. R.: Routine establishment of serial lines of hamster embryo cells transformed by adenovirus type 12. J. nat. Cancer Inst.38, 581–591 (1967).
Schell, K., Jean Maryak, andMargarete Schmidt: Adenovirus transformation, of hamster embryo cells. I. Assay Conditions. Arch. ges. Virusforsch.24, 332–341 (1968).
Eagle, H.: Ammo acid metabolism in mammalian cell cultures. Science130, 432–437 (1959).
Merchant, D. J., R. H. Kahn, andW. H. Murphy, Jr.: Handbook of Cell and Organ Culture, Burgess Publishing Co., Minneapolis, Minnesota.
Todaro, G. J., H. Green, andM. R. Swift: Susceptibility of human diploid fibroblast strain to transformation by SV40 virus. Science153, 1252–1254 (1966).
Defendi, V., andF. Jensen: Oncogenicity of DNA tumor viruses: enhancement after ultraviolet and cobalt-60 radiation. Science157, 703–705 (1967).
Koch, V. G.: Zur unterschiedlichen Infektiosität von isolierter Ribonucleinsäure und intaktem Poliovirus der Gewebekultur. Z. Naturforsch.15b, 656–661 (1960).
Koch, V. G.: Zum Ablauf der Invasion von Virusnucleinsäure in Gewebekulturzellen. Z. Naturforsch.18b, 899–902 (1963).
Latarjet, R.: Inactivation, by UV-, X-, andγ-radiations, of the infecting and transforming capacities of polyoma virus. Virology33, 104–111 (1967).
Author information
Authors and Affiliations
Additional information
This work was supported by the National Institutes of Health, National Institute of Allergy and Infectious Diseases (Contract PH 43-62-200) and the National Cancer Institute (Contract PH 43-64-941).
Rights and permissions
About this article
Cite this article
Schell, K., Maryak, J., Young, J. et al. Adenovirus transformation of hamster embryo cells. Archiv f Virusforschung 24, 342–351 (1968). https://doi.org/10.1007/BF01243075
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF01243075