Skip to main content
Log in

Adenovirus transformation of hamster embryo cells

I. Assay conditions

  • Published:
Archiv für die gesamte Virusforschung Aims and scope Submit manuscript

Summary

Colonies of hamster embryo cells transformed by adenovirus type 12 are characterized by, among other things, the tight packing of small polygonal cells. These cells react selectively with DNA specific reagents. Counting of colonies after reaction with Feulgen or Toluidine blue was possible with the naked eye, and gave counts superior to those in unstained condition under the microscope.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  1. McBride, W. D., andAnna Wiener:In vitro transformation of hamster kidney cells by human adenovirus type 12. Proc. Soc. exp. Biol. (N.Y.)115, 870–874 (1964).

    Google Scholar 

  2. Pope, J. H., andW. P. Rowe: Immunofluorescent studies of adenovirus 12 tumor and of cells transformed or infected by adenovirus. J. exp. Med.120, 577–588 (1964).

    PubMed  Google Scholar 

  3. Rafajko, R. R.: Progress report: Contract PHS43-64-941, p. 11 (1966).

  4. Stich, H. T., G. L. Van Hoosier, andJ. J. Trentin: Viruses and mammalian chromosomes. J. exp. Cell Res.34, 400–403 (1964).

    Google Scholar 

  5. Freeman, A. E., P. H. Black, andR. Wolford: Adenovirus type 12 — rat embryo transformation system. J. Virol.1, 362–367 (1967).

    PubMed  Google Scholar 

  6. Rafajko, R. R.: Routine establishment of serial lines of hamster embryo cells transformed by adenovirus type 12. J. nat. Cancer Inst.38, 581–591 (1967).

    PubMed  Google Scholar 

  7. Schell, K., Jean Maryak, Janice Young, andMargarete Schmidt: Adenovirus transformation of hamster embryo cells. II. Inoculation conditions. Arch. ges. Virusforsch.24, 342–351 (1968).

    PubMed  Google Scholar 

  8. Schell, K., Jean Maryak, andMargarete Schmidt: Adenovirus transformation of hamster embryo cells. III. Maintenance conditions. Arch. ges. Virusforsch.24, 352–360 (1968).

    PubMed  Google Scholar 

  9. Schell, K., Jean Maryak, K. J. Lynch, A. A. Kindurys, andMargarete Schmidt: Adenovirus transformation of hamster embryo cells. IV. Morphological changes induced by various adenoviruses (in press).

  10. Rafajko, R. R.: Production and standardization of adenovirus types 1 to 18 reference antisera. Amer. J. Hyg.79, 310–319 (1964).

    PubMed  Google Scholar 

  11. Hoggan, M. D., H. Blacklow, andW. P. Rowe: Studies of small DNA viruses found in various adenovirus preparations: Physical, biological, and immunological characteristics. Proc. nat. Acad. Sci. (Wash.)55, 1467 (1967).

    Google Scholar 

  12. Atchison, R. W., B. Casto, andW. McD. Hammon: Adenovirus-associated defective virus particles. Science149, 754–755 (1965).

    PubMed  Google Scholar 

  13. Mayor, H. D., andJ. L. Melnick: Small deoxyribonucleic acid-containing viruses (picornavirus group). Nature (Lond.)210, 331–332 (1966).

    Google Scholar 

  14. Kärber, G.: Beitrag zur kollektiven Behandlung pharmakologischer Reihenversuche. Arch. exp. Path. Pharmakol.162, 480–483 (1931).

    Google Scholar 

  15. Eagle, H.: Amino acid metabolism in mammalian cell cultures. Science130, 432–437 (1959).

    PubMed  Google Scholar 

  16. Gurr, E.: Staining: Practical and theoretical. Williams and Wilkins Co., Baltimore, Maryland (1962).

    Google Scholar 

  17. Armstrong, J. A.: Histochemical differentiation of nucleic acids by means of induced fluorescence. Exp. Cell Res.11, 640–643 (1956).

    PubMed  Google Scholar 

  18. Schuemmelfeder, N., K. J. Ebschner, andE. Krogh: Naturwissenschaften44, 467 (1957).

    Google Scholar 

  19. Malinin, G. I., Margarete Schmidt, andK. Schell: Cytochemical observations on adenovirus 12 transformed hamster embryo cells (in preparation).

  20. Korson, R.: A differential stain for nucleic acids. Stain Tech.26, 265–270 (1951).

    Google Scholar 

  21. Todaro, G. J., H. Green, andM. R. Swift: Susceptibility of human diploid fibroblast strains to transformation by SV40 virus. Science153, 1252–1254 (1966).

    PubMed  Google Scholar 

  22. Schell, K.: unpublished observations.

Download references

Author information

Authors and Affiliations

Authors

Additional information

This work was supported by the National Institutes of Health, National Institute of Allergy and Infectious Diseases (Contract PH 43-62-200) and the National Cancer Institute (Contract PH 43-64-941).

Rights and permissions

Reprints and permissions

About this article

Cite this article

Schell, K., Schmidt, M. Adenovirus transformation of hamster embryo cells. Archiv f Virusforschung 24, 332–341 (1968). https://doi.org/10.1007/BF01243074

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01243074

Keywords

Navigation