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A study on the mechanism of polyoma-induced activation of the cellular DNA-synthesizing apparatus

Synchronization by FUdR of virus-induced DNA synthesis

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Summary

Due to the varying length (12 to 30 hours) of the “latent period” (phase 1) in individual cells the onset of the production of polyoma virus (phase 2) in contact-inhibited mouse kidney cells is very asynchronous.

In polyoma-infected mouse tissue cultures cellular and viral DNA synthesis are inhibited efficiently by 5-fluorodeoxyuridine. If DNA synthesis is blocked, the transcription of “late” viral messenger RNA and the formation of capsid protein are inhibited. These inhibitory effects of 5-fluorodeoxyuridine are reversed by thymidine. The “early” events, however, of the infective cycle that lead to the activation of the cellular DNA-synthesizing apparatus take place in the presence of 5-fluorodeoxyuridine and hence do not depend on DNA replication. In the presence of the inhibitor the activated cells accumulate, ready to start DNA synthesis synchronously after the release of the inhibition by thymidine.

The conclusion is reached that in contact-inhibited mouse kidney cells infection with polyoma virus activates specifically the cellular DNA-synthesizing apparatus without triggering the entire mitotic machinery.

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  1. G. Pétursson

    Present address: Institute for Experimental Pathology, University of Iceland, Keldur, Reykjavik, Iceland

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  1. Swiss Institute for Experimental Cancer Research, Lausanne, Switzerland

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Pétursson, G., Weil, R. A study on the mechanism of polyoma-induced activation of the cellular DNA-synthesizing apparatus. Archiv f Virusforschung 24, 1–29 (1968). https://doi.org/10.1007/BF01242898

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  • DOI: https://doi.org/10.1007/BF01242898

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