Summary
It has been confirmed that plaque assays of rhinoviruses may be performed using M-HeLa cells and 30 mM MgCl2. Eighty-seven pools of rhinoviruses grown in human diploid cell strains (HDCS) or organ cultures or as human nasal washings were inoculated into HDCS and M-HeLa cells with 30 mM additional MgCl2 in the medium. Cytopathic effects were produced in HDCS and in M-HeLa cells by 78 pools, in HDCS only by one, in M-HeLa only by one, and in neither cell type by seven pools. A micro metabolic inhibition test was developed which was satisfactory for the titration of rhinoviruses and neutralizing antibody and for identifying rhinovirus strains with specific antisera. Preliminary observations show that rhinovirus type 2 multiplies in suspension cultures of M-HeLa cells. It is concluded that the use of M-HeLa cells with additional MgCl2 instead of HDCS should facilitate the laboratory diagnosis of rhinovirus infections.
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Stott, E.J., Tyrrell, D.A.J. Some improved techniques for the study of rhinoviruses using HeLa cells. Archiv f Virusforschung 23, 236–244 (1968). https://doi.org/10.1007/BF01241896
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DOI: https://doi.org/10.1007/BF01241896