Summary
The first isolation of reovirus type 1 from lambs is reported. The virus was isolated from the nasal and rectal swabs of lambs with respiratory and enteric disease. In the course of the epidemic two phases were observed, the first characterized with relatively mild clinical symptoms and followed by a secondary bacterial complication. The isolated virus is most likely to play a role in inducing the disease. The virus was isolated on foetal lamb kidney cells by inoculating both outgrown cell monolayers and cell suspensions. The latter method was more successful. The isolate was characterized by its cytopathic effect, morphology, resistance to heat, chloroform, sodium deoxycholate, saponin and trypsin and by its heat stability in the presence of divalent cations. Serologically it proved to belong to reovirus type 1.
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Bartha, A., M. Juhász, H. Liebermann, H. Hantschel undP. Schulze: Isolierung und Eigenschaften eines bovinen Herpesvirus von einem Kalb mit respiratorischer Krankheit und Keratokonjunktivitis. Arch. exp. Vet. -Med.21, 615–623 (1967).
Bartha, A., S. Belák, andJ. Benyeda: Trypsin- and heat resistance of some strains of the herpesvirus group. Acta vet. Acad. Sci. hung.19, 97–99 (1969).
Kasza, L.: Isolation and characterization of a reovirus from pigs. Vet. Rec.87, 681–686 (1970).
McFerran, J. B., R. Nelson, J. M. McCracken, andJ. G. Ross: Viruses isolated from sheep. Nature (Lond.)221, 194–195 (1969).
McFerran, J. B., R. Nelson, andJ. K. Clarke: Isolation and characterization of reoviruses isolated from sheep. Arch. ges. Virusforsch.40, 72–81 (1973).
Rhim, J. S., L. E. Jordan, andH. D. Mayor: Cytochemical, fluorescent-antibody and electron microscopic studies on the growth of reovirus (Echo 10) in tissue culture. Virology17, 342–355 (1962).
Rosen, L.: Serological grouping of reoviruses by haemagglutination-inhibition. Amer. J. Hyg.71, 242–249 (1960).
Rosen, L.: Reoviruses Virology Monographs No. (S. Gard, C. Hallauer, andK. F. Meyer, eds.), pp. 73–107. Wien-New York: Springer Verlag, 1968.
Stanley, N. F., P. J. Leak, G. M. Grieve, andD. Perret: The ecology and epidemiology of reovirus. Aust. J. exp. Biol. med. Sci.42, 373–384 (1964).
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Belák, S., Pálfi, V. Isolation of reovirus type 1 from lambs showing respiratory and intestinal symptoms. Archiv f Virusforschung 44, 177–183 (1974). https://doi.org/10.1007/BF01240605
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DOI: https://doi.org/10.1007/BF01240605