Abstract
We have generated an 18-interval contiguous genetic linkage map of human chromosome 4 spanning the entire short arm and proximal long arm. Fifty-seven polymorphisms, representing 42 loci, were analyzed in the Venezuelan reference pedigree. The markers included seven genes (ADRA2C, ALB, GABRB1, GC, HOX7, IDUA, QDPR), one pseudogene (RAF1P1), and 34 anonymous DNA loci. Four loci were represented by microsatellite polymorphisms and one (GC) was expressed as a protein polymorphism. The remainder were genotyped based on restriction fragment length polymorphism. The sex-averaged map covered 123 cM. Significant differences in sex-specific rates of recombination were observed only in the pericentromeric and proximal long arm regions, but these contributed to different overall map lengths of 115 cM in males and 138 cM in females. This map provides 19 reference points along chromosome 4 that will be particularly useful in anchoring and seeding physical mapping studies and in aiding in disease studies.
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Literature cited
Gusella, J.F., Wexler, N.S., Conneally, P.M., Naylor, S.L., Anderson, M.A., Tanzi, R.E., Watkins, P.C., Ottina, K., Wallace, M.R., Sakaguchi, A.Y., Young, A.B., Shoulson, I., Bonilla, E., and Martin, J.B. (1983).Nature 306234–238.
Gilliam, T.C., Healey, S.T., MacDonald, M.E., Wasmuth, J., Stewart, G.D., Roy, J.C., and Gusella, J.F. (1987).Nucleic Acids Res. 151445–1458.
MacDonald, M.E., Anderson, M.A., Gilliam, T.C., Tranebjaerg, L., Carpenter, N.J., Magenis, E., Hayden, M.R., Healy, S.T., Bonner, T.I., and Gusella, J.F. (1987).Genomics 129–34.
Gilliam, T.C., Tanzi, R.E., Haines, J.L., Bonner, T.I., Faryniarz, A.G., Hobbs, W.J., MacDonald, M.E., Cheng, S.V., Folstein, S.E., Conneally, P.M., Wexler, N.S., and Gusella, J.F. (1987).Cell 50565–571.
Allitto, B.A., MacDonald, M.E., Bucan, M., Richards, J., Romano, D., Whaley, W.L., Falcone, B., Ianazzi, J., Wexler, N.S., Wasmuth, J.J., Collins, F.S., Lehrach, H., Haines, J.L., and Gusella, J.F. (1991).Genomics 9104–112.
Tanzi, R.E., Haines, J.L., Watkins, P.C., Stewart, G.D., Wallace, M.R., Hallewell, R., Wong, C., Wexler, N.S., Conneally, P.M., and Gusella, J.F. (1988).Genomics 3129–136.
Haines, J.L., Ozelius, L.J., McFarlane, H., Menon, A., Tzall, S., Martiniuk, F., Hirschhorn, R., and Gusella, J.F. (1990).Genomics 81–6.
Kwiatkowski, D.J., Henske, E.P., Weimer, K., Ozelius, L.J., Gusella, J.F., and Haines, J.L. (1991).Genomics 12229–240.
Anderson, M.A., and Gusella, J.F. (1984).In Vitro 11856–858.
Feinberg, A.P., and Vogelstein, B. (1984).Anal. Biochem. 137266–267.
Weber, J.L., Kwitek, A.E., May, P.E., and Killary, A.M. (1990).Nucleic Acids Res. 1611769–11780.
Dean, M., Lucas-Derse, S., Bolos, A., O'Brien, S.J., Kirkness, E.F., Fraser, C.M., and Goldman, D. (1991).Am. J. Hum. Genet. 49621–626.
Trofatter, J.A., Haines, J.L., and Conneally, P.M. (1986).Am. J. Hum. Genet. 39147–148.
Lander, E.S., Green, P., Abrahamson, J., Barlow, A., Daly, M.J., Lincoln, S.E., and Newburg, L. (1987).Genomics 1174–181.
Haines, J.L. (1992).Genomics 14517–519.
Shiang, R., Murray, J.C., Divelbiss, J.E., Patil, S., Overhauser, J., Wasmuth, J.J., and Buetow, K.H. (1987).Cytogenet. Cell Genet. 46691.
Buckle, V.J., Fujita, N., Ryder-Cook, A.S., Derry, JMJ, Barnard, P.J., Lebo, R.V., Schofield, P.R., Seeburg, P.H., Bateson, A.N., Darlison, M.G., and Barnard, E.A. (1989).Neuron 3647–654.
Kirkness, E.F., Kusiak, J.W., Fleming, J.T., Menninger, J., Gocayne, J.D., Ward, D.C., and Venter, J.C. (1991).Genomics 10985–995.
Buetow, K.H., Shiang, R., Yang, P., Nakamura, Y., Lathrop, G.M., White, R., Wasmuth, J.J., Wood, S., Berdahl, L.D., Leysens, N.J., Ritty, T.M., Wise, M.E., and Murray, J.C. (1991).Am. J. Hum. Genet. 48911–925.
Mills, K.A., Buetow, K.H., Xu, Y., Ritty, T.M., Mathews, K.D., Bodrug, S.E., Wijmenga, C., Balazs, I., and Murray, J.C. (1992).Am. J. Hum. Genet. 51432–439.
Mills, K.A., Buetow, K.H., Xu, Y., Weber, J.L., Altherr, M.R., Wasmuth, J.J., and Murray, J.C. (1992).Genomics 14209–219.
Rouleau, G.A., Haines, J.L., Bazanowski, A., Colella-Crowley, A., Trofatter, J., Wexler, N.S., Conneally, P.M., and Gusella, J.F. (1989).Genomics 41–6.
Haines, J.L., Ozelius, L., St George-Hyslop, P., Wexler, N.S., Gusella, J.F., and Conneally, P.M. (1988).Genet. Epidemiol. 5375–380.
Rouleau, G.A., Bazanowski, A., Gusella, J.F., and Haines, J.L. (1990).Genomics 7313–318.
Tanzi, R.E., Watkins, P.C., Stewart, G.D., Wexler, N.S., Gusella, J.F., and Haines, J.L. (1992).Am. J. Hum. Genet. 50551–558.
Williamson, R., Bowcock, A., Kidd, K., Pearson, P., Schmidtke, J., Ceverha, P., Chipperfield, M., Cooper, D.N., Coutelle, C., Hewitt, J., Klinger, K., Langley, K., Beckman, M., Tolley, M., and Maidak, B. (1991).Cytogenet. Cell Genet. 58231–260.
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Locke, P.A., MacDonald, M.E., Srinidhi, J. et al. A genetic linkage map of the chromosome 4 short arm. Somat Cell Mol Genet 19, 95–101 (1993). https://doi.org/10.1007/BF01233958
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DOI: https://doi.org/10.1007/BF01233958