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Directed isolation of human genes that escape X inactivation

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Somatic Cell and Molecular Genetics

Abstract

Existing methodologies have been combined to produce a directed approach to the isolation of human genes that escape X inactivation. A mouse-human somatic cell hybrid line was established that has an inactive X as its only human chromosome, and nuclear RNA from this cell line was used to construct a cDNA library. Transcribed human sequences were isolated by screening the library with labeled human DNA. The corresponding genomic sequences were isolated in phage or cosmid clones, and exons were identified by detection of transcripts on northern blots. By these means three human loci have been identified that contain genes expressed from an inactive X chromosome. Fluorescence in situ hybridization has been used to map these genes to Xp21.1–22.1, Xp22.1–22.2, and terminal Xp/Yp. One of the three genes (XE45) corresponds to the ZFX gene, while the other two genes (XE7 and XE59) represent novel cloned sequences. Physical and genetic evidence indicate that XE7 is a newly identified pseudoautosomal gene.

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Author notes

  1. Jay Ellison & Larry Shapiro

    Present address: Department of Pediatrics, University of California, 94143, San Francisco, California

  2. Lo-Chung Yu

    Present address: Division of Molecular Cytometry, Department of Laboratory Medicine, University of California, 94143, San Francisco, California

Authors and Affiliations

  1. Howard Hughes Medical Institute, UCLA School of Medicine, Harbor-UCLA Medical Center, 90509, Torrance, California

    Jay Ellison, Pauline Yen & Larry Shapiro

  2. Department of Pediatrics, UCLA School of Medicine, Harbor-UCLA Medical Center, 90509, Torrance, California

    Jay Ellison, Merry Passage, Pauline Yen, T. K. Mohandas & Larry Shapiro

  3. Department of Biological Chemistry, UCLA School of Medicine Harbor-UCLA Medical Center, 90509, Torrance, California

    Larry Shapiro

  4. Biomedical Science Division, Lawrence Livermore National Laboratory, 94550, Livermore, California

    Lo-Chung Yu

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  1. Jay Ellison
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  5. T. K. Mohandas
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Ellison, J., Passage, M., Yu, LC. et al. Directed isolation of human genes that escape X inactivation. Somat Cell Mol Genet 18, 259–268 (1992). https://doi.org/10.1007/BF01233862

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